加味宣白承气汤对肺炎支原体肺炎小鼠肠道菌群及免疫的影响
Effect of Modified Xuanbai Chengqi Decoction on intestinal flora and immunity of mice with mycoplasma pneumoniae pneumonia
- 上海中医药杂志 2023年57卷第7期 页码：5-12
- 作者机构：
1.上海中医药大学附属龙华医院儿科（上海 200032）
- 作者简介：
包春秀，女，硕士研究生，主要从事儿童呼吸系统疾病及肠道微生态研究工作
姜永红，主任医师，硕士研究生导师； E-mail：jyh203225@126.com
- 基金信息：上海市卫健委上海市进一步加快中医药事业发展三年行动计划项目〔ZY（2018-2020）-CCCX-2002-07〕;上海市卫健委“十三五”临床重点专科中医儿科项目(shslczdzk04102);上海市卫健委上海市进一步加快中医药传承创新发展三年行动计划项目〔ZY（2021-2023）-0206-01〕
DOI：10.16305/j.1007-1334.2023.2301044
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包春秀,姜永红.加味宣白承气汤对肺炎支原体肺炎小鼠肠道菌群及免疫的影响[J].上海中医药杂志,2023,57(7):5-12.
BAO Chunxiu,JIANG Yonghong.Effect of Modified Xuanbai Chengqi Decoction on intestinal flora and immunity of mice with mycoplasma pneumoniae pneumonia[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(7):5-12.
包春秀,姜永红.加味宣白承气汤对肺炎支原体肺炎小鼠肠道菌群及免疫的影响[J].上海中医药杂志,2023,57(7):5-12. DOI： 10.16305/j.1007-1334.2023.2301044.
BAO Chunxiu,JIANG Yonghong.Effect of Modified Xuanbai Chengqi Decoction on intestinal flora and immunity of mice with mycoplasma pneumoniae pneumonia[J].Shanghai Journal of Traditional Chinese Medicine,2023,57(7):5-12. DOI： 10.16305/j.1007-1334.2023.2301044.
摘要
目的,2,观察加味宣白承气汤对肺炎支原体肺炎（MPP）小鼠肠道菌群及免疫的影响。,方法,2,选取30只雄性BALB/c小鼠，随机分为空白组、模型组和加味宣白承气汤组，每组10只。除空白组外，其余小鼠以肺炎支原体国际标准株（MPFH）滴鼻造模，加味宣白承气汤组同时以加味宣白承气汤灌胃，连续3 d。第4天取小鼠肺、肠组织，苏木精-伊红（HE）染色观察其病理变化；采用实时荧光定量逆转录聚合酶链式反应（RT-qPCR）法检测小鼠肺组织肺炎支原体脱氧核糖体（MP-DNA）定量及小鼠粪便中丁酸梭菌、黄瘤胃球菌数量；采用酶联免疫吸附试验（ELISA）检测肺泡灌洗液（BALF）中炎症因子［γ干扰素（IFN-γ）、转化生长因子-β（TGF-β）、白介素-6（IL-6）、白介素-10（IL-10）、白介素-17（IL-17）］水平及粪便中分泌型免疫球蛋白A（sIgA）水平；Western blot法检测肺、肠组织Toll样受体4（TLR4）蛋白表达。,结果,2,①模型组肺、肠组织病理切片可见炎性浸润最为严重，加味宣白承气汤组炎性浸润较轻。 ②与模型组比较，加味宣白承气汤组MP-DNA含量显著下降（,P,<,0.05）。③与空白组比较，模型组及加味宣白承气汤组黄瘤胃球菌、丁酸梭菌均显著减少（,P,<,0.05）；与模型组比较，加味宣白承气汤组黄瘤胃球菌、丁酸梭菌均显著增加（,P,<,0.05）。④与空白组比较，模型组及加味宣白承气汤组sIgA含量显著减少（,P,<,0.05）；与模型组比较，加味宣白承气汤组sIgA含量显著增加（,P,<,0.05）。⑤与空白组比较，模型组、加味宣白承气汤组BALF中IFN-γ、TGF-β、IL-6、IL-10、IL-17水平均显著升高（,P,<,0.05）；与模型组比较，加味宣白承气汤组上述指标均显著降低（,P,<,0.05）。⑥黄瘤胃球菌、丁酸梭菌与sIgA含量均呈正相关（,P,<,0.05）。⑦与空白组比较，模型组、加味宣白承气汤组肺、肠组织中TLR4蛋白表达量均显著增加（,P,<,0.05）；与模型组比较，加味宣白承气汤组肺、肠组织中TLR4蛋白表达量均显著降低（,P,<,0.05）。,结论,2,加味宣白承气汤可下调TLR4的表达，降低MPP炎症因子水平，有良好的抗炎作用；同时该方能有效修复肺、肠组织损伤，增加肠道黄瘤胃球菌及丁酸梭菌菌群数量，促进肠道黏膜sIgA水平升高。
Abstract
Objective,2,To observe the effect of Modified Xuanbai Chengqi Decoction （MXBCQD） on intestinal flora and immunity of mice with mycoplasma pneumoniae pneumonia （MPP）.,Methods,2,Thirty male BALB/c mice were randomly divided into blank group （,n,=10）， model group （,n,=10） and MXBCQD group （,n,=10）. Except for the blank group， the other mice were modeled by nasal drip of mycoplasma pneumonia （MP） international standard strain， and the mice in the MXBCQD group were fed with MXBCQD for 3 days. On the 4th day， the lungs and intestines of mice were taken and the pathological changes were observed by hematoxylin-eosin （HE） staining， and the quantity of MP-DNA in lung tissue and the numbers of Clostridium butyricum and rumen cocci in feces were detected by real-time fluorescence quantitative reverse transcriptase polymerase chain reaction （RT-qPCR）. The levels of inflammatory factors such as interferon gamma （IFN-γ）， transforming growth factor β （TGF-β）， interleukin 6 （IL-6）， interleukin 10 （IL-10）， interleukin 17 （IL-17） in bronchoalveolar lavage fluid （BALF） and secretory immunoglobulin A （sIgA） in feces were detected by enzyme linked immunosorbent assay （ELISA）. The protein expression of Toll-like receptor 4 （TLR4） in the lung and intestine was detected by Western blot method.,Results,2,①Pathological sections of the lung and intestine showed that the inflammatory infiltration in the model group was the most severe， while that in the MXBCQD group was milder. ②Compared with the model group， the content of MP-DNA in MXBCQD group was decreased significantly （,P,<,0.05）. ③Compared with the blank group， the rumen cocci and Clostridium butyricum in the model group and MXBCQD group were decreased significantly （,P,<,0.05）， and the numbers of the rumen cocci and Clostridium butyricum in MXBCQD group were increased significantly compared with the model group （,P,<,0.05）. ④Compared with the blank group， the sIgA contents in the model group and MXBCQD group were decreased significantly （,P,<,0.05）， while compared with the model group， the sIgA content in MXBCQD group was significantly increased （,P,<,0.05）. ⑤Compared with the blank group， the levels of IFN-γ， TGF-β， IL-6， IL-10， and IL-17 in BALF of the model group and MXBCQD group were increased significantly （,P,<,0.05）. Compared with the model group， the levels of those indicators were decreased significantly in MXBCQD group （,P,<,0.05）. ⑥Gastrococcus flavum and Clostridium butyricum were positively correlated with sIgA content （,P,<,0.05）. ⑦Compared with the blank group， the expression levels of TLR4 protein in the lung and intestinal tissues of the model group and the MXBCQD group were increased significantly （,P,<,0.05）. Compared with the model group， the expression levels of TLR4 protein in lung and intestinal tissues of the MXBCQD group were decreased significantly （,P,<,0.05）.,Conclusion,2,MXBCQD can down-regulate the expression of TLR4 and reduce the level of MPP inflammatory factors， which has a good anti-inflammatory effect； at the same time， it can effectively repair lung and intestinal tissue injury， increase the number of intestinal rumen cocci and Clostridium butyricum， and promote the level of sIgA in intestinal mucosa.
关键词
肺炎支原体肺炎加味宣白承气汤经典名方肠道菌群小鼠模型中药研究
Keywords
mycoplasma pneumoniae pneumoniamodified Xuanbai Chengqi DecoctionChinese classic formulasintestinal floramouse modeltraditional Chinese herbal medicine research
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