Objective,2,To explore the implementation pattern of “three zones and two passages” alteration and localized transformation， organization and coordination management required for nosocomial infection prevention and control during the emergency transformation of a tertiary general hospital into a designated hospital for COVID-19.,Methods,2,With the system methodology of “Wuli⁃Shili⁃Renli （WSR）” and the goal of “zero infection among medical and nursing staff”， the characteristics of current hospital zone division were carefully studied， and the scheme of real-time data dynamic management and effective allocation of emergency resources was constructed from the aspects of reasonable zone division and passage planning， clear signs of nosocomial infection prevention and control， establishment of nosocomial infection prevention and control process， and three-zone linkage team-building for nosocomial infection prevention and control.,Results,2,With the intervention of a series of nosocomial infection prevention and control measures， the prevention and control effect was satisfactory and zero nosocomial infection was achieved.,Conclusion,2,The practice and exploration of WSR approach for nosocomial infection prevention and control in a tertiary general hospital converted to a designated hospital for COVID-19 can be used as a reference and a paradigm for dealing with public health emergencies such as sudden unexpected infectious diseases.