Objective,2,To explore the regulatory effect of serum containing traditional Chinese medicine compound with the function of tonifying the kidney and disinhibiting dampness on neutrophilic alkaline phosphatase 6（NALP6） signal transduction pathway in fibroblast-like synoviocytes of gouty arthritis （GA） rats.,Methods,2,The gouty arthritis model was established by adding sodium urate microcrystalline into the rat fibroblast synovial cell culture medium. The drug containing serum which is most suitable for the survival of rat synovial cells was added into the culture medium for cell experiment. The cells were divided into the blank group （normal synoviocytes）， the model group （synoviocytes + sodium urate） and the treatment group （synoviocytes + sodium urate + drug-containing serum） and each group carried on the corresponding intervention. The expression of ,NALP6, mRNA in synovial cells was detected by RT-PCR， the expression of NALP6， NF-κB， IκB by Western blot， the Caspase-1 activity by Colorimetric Method and IL-1β content in synovial cells of each group by ELISA.,Results,2,In the model group， compared with the blank group， the expressions of ,NALP6, mRNA， NALP6 and NF-κB proteins were up-regulated， while the expression of IκB protein was down-regulated. Caspase-1 activity and IL-1β content were increased（all ,P,<,0.05）. In the treatment group， compared with the model group， the serum containing TCM compound could significantly inhibit the expressions of ,NALP6 ,mRNA， NALP6 and NF-κB proteins， inhibit the activity of Caspase-1， reduce the content of IL-1β， and stimulate the expression of IκB protein in fibroblast synovial cells（all ,P,<,0.05）.,Conclusion,2,Blocking the “NALP6-Caspase-1-IL-1β” inflammatory signal pathway may be one of the most important mechanisms for preventing and treating the gouty arthritis through tonifying the kidney and disinhibiting dampness therapy.