Objective,2,To investigate and compare the surface electromyography （sEMG） characteristics of the main force-generating muscles of the upper limbs of the operator conducting two different types of Shi’s cervical rotatory manipulation （cervical rotation manipulation and cervical rotation-traction manipulation）.,Methods,2,An operator proficient in Shi’s cervical rotatory manipulation was selected to perform 2 different types of cervical rotatory manipulation， twice for each type， on 20 subjects in the same anatomical position. The sEMG signal data of the brachioradialis muscle， biceps brachii muscle， triceps brachii muscle， deltoid muscle， supraspinatus muscle and pectoralis major muscle on the right side of the operator were collected， and the sEMG characteristics of the upper limb force-generating muscles of the operator conducting cervical rotation manipulation and cervical rotation-traction manipulation were analyzed and summarized.,Results,2,①During the operation of cervical rotation manipulation， biceps brachii was the first to be activated， followed by deltoid， supraspinatus， triceps brachii， brachioradialis and pectoralis major， and the sequence of muscle discharge duration was consistent with the activation sequence. The muscle with the largest contribution rate was biceps brachii， and the muscle with the smallest contribution rate was pectoralis major. ②During the operation of cervical rotation-traction manipulation， brachioradialis muscle was the first to be activated， followed by triceps brachii， biceps brachii， deltoid， supraspinatus and pectoralis major. The sequence of muscle discharge duration was brachioradialis， biceps， triceps， deltoid， supraspinatus， and pectoralis major. Brachioradialis muscle had the longest duration of muscle discharge， biceps brachii and triceps brachii had almost equal duration， and pectoralis major muscle had the shortest duration. The brachioradialis muscle had the largest contribution rate， and the pectoralis major muscle had the smallest contribution rate. ③Compared with the values under the operation of cervical rotation-traction manipulation， the values of integrated EMG （iEMG） and （root mean square） RMS of brachioradialis muscle were lower （,P,<,0.01）， the values of iEMG of biceps brachii， deltoid， supraspinatus， pectoralis major and triceps brachii were higher （,P,<,0.05， ,P,<,0.01）， and the values of RMS of biceps brachii， deltoid， pectoralis major and triceps brachii were higher （,P,<,0.05， ,P,<,0.01） under the operation of cervical rotation manipulation.,Conclusion,2,The biceps brachii is the main muscle for the practice of cervical rotation manipulation， and the brachioradialis is the main muscle for the practice of cervical rotation-traction manipulation. Therefore， the muscle training for brachioradialis and biceps brachii can provide certain favorable conditions for manipulation.