1.上海中药标准化研究中心(上海 201203)
2.上海中医药大学中药研究所,中药标准化教育部重点实验室(上海 201203)
3.福州梅峰制药厂(福建 福州 350007)
方洪,女,硕士研究生,主要从事中药质量标准研究工作
谷丽华,副研究员,硕士研究生导师; E-mail:lhgu@shutcm.edu.com
扫 描 看 全 文
方洪,陈夕梅,谷丽华,等.中成药术苓健脾胶囊的薄层色谱鉴别优化研究[J].上海中医药杂志,2022,56(7):102-106.
FANG Hong,CHEN Ximei,GU Lihua,et al.Study on improvement of TLC identification of Zhuling Jianpi Capsule[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(7):102-106.
方洪,陈夕梅,谷丽华,等.中成药术苓健脾胶囊的薄层色谱鉴别优化研究[J].上海中医药杂志,2022,56(7):102-106. DOI: 10.16305/j.1007-1334.2022.2008052.
FANG Hong,CHEN Ximei,GU Lihua,et al.Study on improvement of TLC identification of Zhuling Jianpi Capsule[J].Shanghai Journal of Traditional Chinese Medicine,2022,56(7):102-106. DOI: 10.16305/j.1007-1334.2022.2008052.
目的,2,完善优化术苓健脾胶囊的薄层色谱鉴别方法。,方法,2,将术苓健脾胶囊内容物依次用正己烷和甲醇超声提取,提取液分别用于制剂中白术的鉴别和制剂中白芍、黄柏和陈皮的鉴别,取部分甲醇提取液过滤、蒸干,加水溶解,通过D101型大孔吸附树脂柱(内径2 cm,树脂高15 cm),分别用10%甲醇、50%甲醇、80%甲醇和90%甲醇依次洗脱,取50%甲醇洗脱液,用于制剂中乌药和防风的鉴别;取80%甲醇洗脱液,用于制剂中甘草的鉴别;取90%甲醇洗脱液,用于制剂中厚朴的鉴别。,结果,2,一次取样制备5份供试品溶液,用于制剂中8味药材的薄层色谱鉴别。白芍以芍药苷为指标,乌药以去甲异波尔定为指标,防风以升麻素苷和5-,O,-甲基维斯阿米醇苷为指标,均以氯仿-甲醇(4∶1)为展开剂;黄柏以盐酸小檗碱为指标,陈皮以橙皮苷为指标,均以氯仿-甲醇-水(37∶17∶5)的下层溶液为展开剂;厚朴以厚朴酚及和厚朴酚为指标,甲苯-甲醇(10∶1)为展开剂,甘草以甘草苷和甘草酸单铵盐为指标,乙酸乙酯-甲酸-冰醋酸-水(15∶1∶1∶2)为展开剂。防风、乌药和甘草为新增薄层色谱鉴别项,厚朴、黄柏和陈皮的薄层色谱鉴别项得到优化。,结论,2,采用一次系统试验完成术苓健脾胶囊多味药材薄层色谱鉴别项的供试品溶液制备,多味药材使用相同展开剂,操作方法更加简便,重现性和分离度提高,更有助于术苓健脾胶囊的质量控制。
Objective,2,To improve the TLC identification of Zhuling Jianpi Capsule.,Methods,2,The supernatants of the inner content of Zhuling Jianpi Capsule via successive ultrasonication with ,n,-hexane following with mehanol were separately used for the identification of Atractylodis Macrocephalae Rhizoma and for the identifications of Paeoniae Radix Alba, Phellodendri Chinensis Cortex and Citri Reticulatae Pericarpium in Zhuling Jianpi Capsule. The residual methanol filtrate was evaporated to dryness and dissolved with water, applied to D101 macroporous resin column (inner diameter 2 cm, column length 15 cm), eluted successively with 10% methanol, 50% methanol, 80% methanol and 90% methanol. The 50% methanol eluate was used for identification of Linderae Radix and Saposhnikoviae Radix, 80% methanol eluate was used for identification of Glycyrrhizae Radix et Rhizoma, and 90% methanol eluate for identification of Magnoliae Officinalis Cortex.,Results,2,Eight TLC identification methods were accomplished, and three TLC identification methods for Saposhnikoviae Radix, Linderae Radix and Glycyrrhizae Radix et Rhizomawere were newly established and three TLC identification methods for Magnoliae Officinalis Cortex, Phellodendri Chinensis Cortex and Citri Reticulatae Pericarpium were improved. Paeoniflorin was used as the marker of Paeoniae Radix Alba, norisoboldine as the marker of Linderae Radix, prim-O-glucosylcimifugin and 5-O-methylvisammioside as the markers of Saposhnikoviae Radix, and the mixture of chloroform and methanol (4∶1) was used as the same mobile phase for three of them; berberine hydrochloride was used as the marker of Phellodendri Chinensis Cortex and hesperidin as the marker of Citri Reticulatae Pericarpium, and the low-layer solution of the mixture of chloroform, methanol and water (37∶17∶5) was used as the same mobile phase for both of them; Magnolol and honokiol were used as the markers of Magnoliae Officinalis Cortex and the mixture of toluene and methanol (10∶1) as the mobile phase; liquiritin and monoammonium glycyrrhizinate were used as the markers of Glycyrrhizae Radix et Rhizoma, and the mixture of ethyl acetate, formic acid, glacial acetic acid, and water (15∶1∶1∶2) as the mobile phase.,Conclusion,2,A systematic sample test solution preparation strategy is implemented for concurrently preparing 4 test solutions for eight TLC identifications, the modified TLC identification methods are more suitable to control the quality of Zhuling Jianpi Capsule.
肠易激综合征术苓健脾胶囊薄层色谱质量标准中药研究
irritable bowel syndromeZhuling Jianpi CapsuleTLC identificationquality standardtraditional Chinese herbal medicine research
周跃华,路金才,周娟,等. 关于中药新药复方制剂“整体鉴别”新模式的思考[J]. 中草药,2021, 52(8): 2199-2204.
刘宇. 几种复方中成药质量标准的研究[D]. 扬州:扬州大学,2016.
吕玥,汤湧,李立言,等. 中药复方制剂质量控制体系的研究进展[J]. 中国中西医结合外科杂志,2020, 26(1): 170-172.
刘爱香. 中药及中成药薄层色谱鉴别检验中的问题分析[J]. 光明中医,2018, 33(22): 167-169.
国家药典委员会.中华人民共和国药典(2020年版):一部[M].北京:中国医药科技出版社,2020.
SANTIAGO M, STROBEL S. Thin layer chromatography[J]. Methods Enzymol, 2013(533): 303-324.
MINYUK G S, SOLOVCHENKO A E. Express analysis of microalgal secondary carotenoids by TLC and UV-Vis spectroscopy[J]. Methods Mol Biol, 2018(1852): 73-95.
刘世欣,陈良妮,程雪梅,等.肾康宁胶囊的薄层色谱定性鉴别质量控制方法研究[J].上海中医药杂志,2021, 55(10): 66-72.
王金淼,池军,江雪,等. 皂角刺药材和饮片质量标准研究[J]. 中国中药杂志,2021, 46(17): 4417-4423.
马金鑫. 术苓健脾胶囊治疗腹泻型肠易激综合征的临床疗效观察[D]. 延安:延安大学,2019.
0
浏览量
732
下载量
0
CSCD
2
CNKI被引量
关联资源
相关文章
相关作者
相关机构