针药结合调控子宫内膜异位症大鼠内膜组织ERα、ERβ的作用
Effect of acupuncture and moxibustion with traditional Chinese medicine in regulating endometrial tissue ERα/ERβ in rats with endometriosis
- 上海中医药杂志 2021年55卷第11期 页码：59-65
- 作者机构：
1.上海中医药大学附属岳阳中西医结合医院（上海 200437）
2.上海中医药大学附属龙华医院（上海 200030）
- 作者简介：
吴楚婷，女，硕士研究生，主要从事针灸防治妇科疾病的临床与实验研究工作
张春雁，博士，副主任医师；E-mail：zh_ch_yan@163.com
- 基金信息：国家自然科学基金项目(81403429);上海市科委科研计划项目(19401934500);上海申康医院发展中心临床三年行动计划项目(SHDC2020CR3093B);上海中医药大学“杏林百人”计划杏林学者项目
DOI：10.16305/j.1007-1334.2021.2108116
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吴楚婷, 李铭旸, 张晓云, 等. 针药结合调控子宫内膜异位症大鼠内膜组织ERα、ERβ的作用[J]. 上海中医药杂志, 2021,55(11):59-65.
Chuting WU, Mingyang LI, Xiaoyun ZHANG, et al. Effect of acupuncture and moxibustion with traditional Chinese medicine in regulating endometrial tissue ERα/ERβ in rats with endometriosis[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(11):59-65.
吴楚婷, 李铭旸, 张晓云, 等. 针药结合调控子宫内膜异位症大鼠内膜组织ERα、ERβ的作用[J]. 上海中医药杂志, 2021,55(11):59-65. DOI： 10.16305/j.1007-1334.2021.2108116.
Chuting WU, Mingyang LI, Xiaoyun ZHANG, et al. Effect of acupuncture and moxibustion with traditional Chinese medicine in regulating endometrial tissue ERα/ERβ in rats with endometriosis[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(11):59-65. DOI： 10.16305/j.1007-1334.2021.2108116.
摘要
目的,2,研究针药结合调控子宫内膜异位症（内异症）雌激素受体的作用。,方法,2,50只SD大鼠按体质量随机分为假手术组、模型组、针灸组、中药组和针药组，每组10只；建立大鼠内异症模型；针灸组针刺“血海”“三阴交”，艾灸“关元”，中药组给予加味没竭片0.9%NaCl溶液混悬液灌胃，针药组给予上述2种方法综合治疗，假手术组、模型组进行固定针灸并给予等量0.9%NaCl溶液灌胃。治疗30 d后，测量各组大鼠异位囊肿直径；取正常子宫内膜、在位及异位内膜组织进行免疫组织化学检测，比较不同组织中雌激素受体α（ERα）、雌激素受体β（ERβ）的表达。,结果,2,与模型组比较，针灸组、中药组和针药组异位囊肿直径显著缩小（,P,<,0.05），针药组异位囊肿直径显著小于针灸组和中药组（,P,<,0.05）。针灸组和针药组在位及异位内膜ERα表达显著高于模型组（,P,<,0.01）和中药组（,P,<,0.01），针药组在位内膜ERα表达高于针灸组（,P,<,0.05）。针灸组、中药组、针药组在位及异位内膜ERβ表达均显著低于模型组（,P,<,0.01），针药组在位及异位内膜ERβ表达均低于中药组（,P,<,0.01）。内异症大鼠在位及异位内膜组织ERα/ERβ比率均较低，其中针药组大鼠在位及异位内膜组织中ERα/ERβ比率高于其余各组。,结论,2,针药结合可能通过上调在位及异位内膜组织中ERα表达、下调在位及异位内膜组织中ERβ表达，使其趋于正常子宫内膜水平，进而达到治疗大鼠内异症的效应。
Abstract
Objective,2,To research the effect of acupuncture and moxibustion with Chinese medicine in regulating the estrogen receptor in endometriosis.,Methods,2,Fifty SD rats were randomly divided into sham operation group, model group, acupuncture and moxibustion group, Chinese medicine group, acupuncture and moxibustion with Chinese medicine group (AC group in brief), 10 rats in each group. Endometriosis rat models were established in advance. The acupuncture and moxibustion group received acupuncture at Xuehai (SP10) and Sanyinjiao (SP6) and moxibustion at Guanyuan (CV4); the Chinese medicine group was treated with 0.9% NaCl suspension of Jiawei Mojie tablet by oral gavage; the AC group was treated by the above two methods simultaneously. The sham operation group and the model group were fixed and given of the same dosage of 0.9% NaCl by oral gavage. After 30 days of treatment, the diameters of ectopic cysts in five groups were measured. Normal endometrial, eutopic and ectopic endometrial tissues were taken for immunohistochemical detection and the expressions of estrogen receptor α (ERα) and estrogen receptor β (ERβ) in different tissues were compared.,Results,2,The diameter of ectopic cyst in acupuncture and moxibustion group, Chinese medicine group, and AC group was significantly smaller than that in model group (,P,<,0.05). The diameter of ectopic cyst in AC group was significantly smaller than that both in acupuncture and moxibustion group and Chinese medicine group (,P,<,0.05). The ERα expression in ectopic and eutopic endometrium of acupuncture and moxibustion group and AC group was significantly higher than that of model group (,P,<,0.01) and Chinese medicine group (,P,<,0.01). The ERα expression in eutopic endometrium of AC group was higher than that of acupuncture and moxibustion group (,P,<,0.05). The ERβ expression in ectopic and eutopic endometrium of acupuncture and moxibustion group, Chinese medicine group and AC group was lower than that of model group (,P,<,0.01). The ERβ expression in ectopic and eutopic endometrium of AC group was significantly lower than that of Chinese medicine group (,P,<,0.01). ERα/ERβ ratio was lower in the ectopic and eutopic endometrium of EMS rats. ERα/ERβ ratio in the ectopic and eutopic endometrium of AC group was the highest among all the groups.,Conclusion,2,The combination of acupuncture and moxibustion with Chinese medicine may achieve the treatment effect of rats’ endometriosis through up-regulating the expression of ERα protein and down-regulating the expression of ERβ protein in eutopic and ectopic endometrium to the normal level.
关键词
子宫内膜异位症疾病模型大鼠针药结合雌激素受体α雌激素受体β
Keywords
endometriosisacupuncture and moxibustion with Chinese medicineestrogen receptor αestrogen receptor β
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