Objective,2,To explore the effect of resveratrol on IL-1β induced apoptosis of growth plate chondrocytes, and to screen the optimal concentration of resveratrol and verify the effect of resveratrol on delaying the closure of the rat epiphysis and promoting the growth of long bones.,Methods,2,The growth plate chondrocytes of 4-week-old SD rats were isolated and cultured. The cells were identified by type Ⅱ collagen immunofluorescence. The chondrocytes were randamly divided into 9 groups: normal control group(ordinary medium), model control group(containing 20 ng/mL IL-1β), positive control group(containing 20 ng/mL IL-1β and 5 μmol/L letrozole), DMSO control group(DMSO added with resveratrol in culture medium), experimental group 1(only 40 μmol/L resveratrol), experimental group 2 to experimental group 5(containing 20 ng/mL IL-1 β and 10, 20, 40, 60 μmol/L resveratrol, respectively). MTT colorimetric method was used to screen the optimal concentration of resveratrol to inhibit chondrocyte apoptosis and promote cell proliferation. Flow cytometry was used to detect the optimal concentration of resveratrol to inhibit chondrocyte apoptosis. The effects of resveratrol on epiphyseal closure and tibial growth of tibial plateau in rats were observed by intragastric administration of appropriate concentration of resveratrol.,Results,2,Chondrocytes of growth plate were isolated and cultured. MTT assay results showed that compared with the normal control group, the proliferation ability of the model control group was significantly decreased(,P,<,0.05); compared with the model control group, letrozole and resveratrol at different concentrations could antagonize the apoptosis induced by IL-1β, and the difference was statistically significant(,P,<,0.01). The intervention effect of resveratrol was better than letrozole, and the optimal concentration was 40 μmol/L. The results of flow cytometry showed that the apoptosis rate of the model control group was significantly higher than that of the normal control group(,P,<,0.05). Resveratrol at 40 μmol/L could reduce the apoptosis rate of rat growth plate chondrocytes induced by IL-1β, and the intervention effect was better than letrozole. Resveratrol could delay the epiphyseal closure of tibial plateau and promote the growth of tibia in rats by intragastric administration of resveratrol at 100 mg/kg.,Conclusion,2,Resveratrol at 40 μmol/L can inhibit the apoptosis of chondrocytes induced by IL-1β significantly and gavage of 100 mg/kg resveratrol in rats can delay the epiphyseal closure of the tibial plateau and promote bone growth in the developmental stage by prolonging the time of bone growth.