升清胶囊对胆固醇结石小鼠核受体及三磷酸腺苷结合盒（ABC）转运蛋白影响的研究

沈亮; 胡诚; 章学林; 顾宏刚; 张静喆; 梁晓强

doi:10.16305/j.1007-1334.2021.2103068

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升清胶囊对胆固醇结石小鼠核受体及三磷酸腺苷结合盒（ABC）转运蛋白影响的研究

实验研究 | 更新时间：2022-08-03

- 升清胶囊对胆固醇结石小鼠核受体及三磷酸腺苷结合盒（ABC）转运蛋白影响的研究
- Research on effect of Shengqing Capsule on nuclear receptors and ATP binding cassette transporters in cholesterol gallstone mice
- 上海中医药杂志 2021年55卷第8期页码：88-93
- 作者机构：
  
  1.上海中医药大学附属龙华医院（上海　200032）
  2.上海中医药大学科技实验中心（上海　201203）
- 作者简介：
  
  沈亮，男，硕士，主治医师，主要从事中医药防治外科常见疾病的临床与基础研究工作
  梁晓强，博士，副研究员；E-mail：jennielxq@126.com
- 基金信息：
  
  国家中医药管理局全国中医学术流派传承工作室第二轮建设项目(国中医药人教函〔2019〕62号);上海市卫健委资助项目(2018JP012);上海市卫健委进一步加快中医药事业发展三年行动计划项目(ZY〔2018-2020〕-CCCX-1007)
- DOI：10.16305/j.1007-1334.2021.2103068
  中图分类号：
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沈亮, 胡诚, 章学林, 等. 升清胶囊对胆固醇结石小鼠核受体及三磷酸腺苷结合盒（ABC）转运蛋白影响的研究[J]. 上海中医药杂志, 2021,55(8):88-93.

Liang SHEN, Cheng HU, Xuelin ZHANG, et al. Research on effect of Shengqing Capsule on nuclear receptors and ATP binding cassette transporters in cholesterol gallstone mice[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(8):88-93.
沈亮, 胡诚, 章学林, 等. 升清胶囊对胆固醇结石小鼠核受体及三磷酸腺苷结合盒（ABC）转运蛋白影响的研究[J]. 上海中医药杂志, 2021,55(8):88-93. DOI： 10.16305/j.1007-1334.2021.2103068.

Liang SHEN, Cheng HU, Xuelin ZHANG, et al. Research on effect of Shengqing Capsule on nuclear receptors and ATP binding cassette transporters in cholesterol gallstone mice[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(8):88-93. DOI： 10.16305/j.1007-1334.2021.2103068.

摘要

目的,2,探讨升清胶囊对胆固醇结石模型小鼠核受体及三磷酸腺苷结合盒（ABC）转运蛋白表达的影响。,方法,2,将30只C57BL/6J小鼠随机分为正常组、模型组和升清胶囊组，每组10只；模型组和升清胶囊组采用喂养高脂致石饲料连续7周诱导胆固醇结石模型，正常组喂养正常饲料；升清胶囊组在造模的同时给予相应药物灌胃，正常组和模型组给予等量0.9%NaCl溶液灌胃。各组均于造模结束后取材，观察成石率，HE染色观察胆囊和肝脏大体形态变化，酶比色法检测血清及肝脏TG、TC、TBA含量，Real-time PCR和Western blot法分别检测肝脏,LXR,-α、,FXR,、,ABCA,1、,ABCG,5、,ABCG,8 mRNA及蛋白表达。,结果,2,①与正常组比较，模型组成石率升高（,P,<,0.05），胆囊和肝脏大体形态学存在不同程度损伤；与模型组比较，升清胶囊组成石率降低（,P,<,0.05），胆囊和肝脏大体形态学均有不同程度改善。②与正常组比较，模型组血清和肝脏TC、TG含量升高，肝脏TBA含量降低，肝脏,LXR,-α、,FXR,、,ABCA,1、,ABCG,5、,ABCG,8 mRNA及蛋白表达均升高（,P,<,0.05，,P,<,0.01）；与模型组比较，升清胶囊组血清和肝脏TC、TG含量降低，,LXR,-α、,FXR,、,ABCG,5、,ABCG,8 mRNA及蛋白表达均降低，ABCA1蛋白表达降低（,P,<,0.05，,P,<,0.01）。,结论,2,升清胶囊可能是通过下调LXR/FXR及其直接靶基因ABC转运蛋白来降低C57小鼠结石成石率。

Abstract

Objective,2,To study effect of Shengqing Capsule on nuclear receptors and ATP Binding Cassette (ABC) Transporters in cholesterol gallstone mice.,Methods,2,30 C57BL/6J mice were randomly divided into normal group, model group and Shengqing Capsule group, with 10 mice in each group. The cholesterol gallstone model group was developed by feeding high-fat lithogenic diet for 7 consecutive weeks, while the normal group was fed with normal diet. The Shengqing Capsule group was administered drug (0.355 g/kg·d) by gavage when modeling, while the normal group and model group were administered the same amount of normal saline by gavage. After modeling, the stone formation rate in each group was observed. The gross morphological changes of gallbladder and liver were assessed by the H,&,E staining, the levels of Triglycerides (TG) , total cholesterol (TC) , and total bile acids (TBA) in serum and liver were measured by GPO-PAP. Real time-PCR and western blot were performed to detect the mRNA and protein expression levels of ,LXR,-α,FXR,ABCA,1,ABCG,5,ABCG,8 in the liver respectively.,Results,2,①Compared with the normal group, the stone formation rate increased (,P,<,0.05) , and the gross morphology of gallbladder and liver was damaged in various degrees in the model group. The stone formation rate reduced (,P,<,0.05) , the gross morphology of gallbladder and liver was improved in various degrees of the Shengqing Capsule group compared with those of the model group. ②Compared with the normal group, the levels of TC and TG in serum and liver increased, but the level of TBA in liver decreased, the mRNA and protein expressions of ,LXR,-α,FXR,ABCA,1,ABCG,5,ABCG,8 in liver increased in the model group (,P,<,0.05,P,<,0.01) . Compared with the model group, the leuel of TC and TG in serum and liver significantly decreased, the mRNA of ,LXR,-α,FXR,ABCG,5 and ,ABCGB, decreasecl, and the protein expression of ,ABCA,1 decreased in the Shenqing Capsule group (,P,<,0.05,P,<,0.01),Conclusion,2,Shengqing Capsule may down-regulate LXR-α/FXR and its direct gene ATP-binding cassette transports to reduce the stone formation rate in C57 mice.

关键词

升清胶囊胆固醇结石小鼠核受体LXR-α/FXRABC转运蛋白

Keywords

shengqing Capsulecholesterol gallstonecholesterol-bile acid metabolic chainnuclear receptors LXR-α/FXRthe ATP binding cassette transporters

references

陈希纲，刘家奇，彭民浩，等. 胆石病临床流行病学调查——附8 585例分析[J]. 中华普通外科杂志，2002, 17(2): 99-101.

黄志强. 胆道外科的现状与未来[J]. 中国实用外科杂志，1999, 19(1): 17-18.

宋华荣，朱培庭，张静喆，等. 胆石净治疗慢性胆道感染、胆石病的疗效观察[J]. 上海中医药杂志，2002,36(8): 13-14.

朱培庭，王以实，宋荣华，等. 清胆胶囊治疗慢性胆道感染及胆石病的临床疗效[J]. 中国中西医结合外科杂志，1997, 3(3): 152-154.

朱培庭，张静喆，王以实，等. 清胆胶囊治疗慢性胆道感染、胆石病的临床研究[J]. 上海中医药大学学报，1999, 13(1): 23-26.

陈奇. 中药药理实验方法学[M]. 北京：人民卫生出版社，1994: 206-232.

ROSALES R, ROMERO M R, VAQUERO J, et al. FXR-dependent and -independent interaction of glucocorticoids with the regulatory pathways involved in the control of bile acid handling by the liver[J]. Biochem Pharmacol, 2013, 85(6): 829-838.

KONG B, WANG L, CHIANG J Y L, et al. Mechanism of tissue-specific farnesoid X receptor in suppressing the expression of genes in bile-acid synthesis in mice[J]. Hepatology, 2012, 56(3): 1034-1043.

LI X L, YEH V, MOLTENI V. Liver X receptor modulators: a review of recently patented compounds (2007-2009)[J]. Expert Opin Ther Pat, 2010, 20(4): 535-562.

KLAPPE K, HUMMEL I, HOEKSTRA D, et al. Lipid dependence of ABC transporter localization and function[J]. Chem Phys Lipids, 2009, 161(2): 57-64.

KUO K K, SHIN S J, CHEN Z C, et al. Significant association of ABCG5 604Q and ABCG8 D19H polymorphisms with gallstone disease[J]. Br J Surg, 2008, 95(8): 1005-1011.

SABEVA N S, LIU J J, GRAF G A. The ABCG5 ABCG8 sterol transporter and phytosterols: implications for cardiometabolic disease[J]. Curr Opin Endocrinol Diabetes Obes, 2009, 16(2): 172-177.

JIANG Z Y, PARINI P, EGGERTSEN G, et al. Increased expression of LXR alpha, ABCG5, ABCG8, and SR-BI in the liver from normolipidemic, nonobese Chinese gallstone patients[J]. J Lipid Res, 2008, 49(2): 464-472.

韩天权，姜翀弋，张圣道. 胆固醇结石病的病因和机制研究进展[J]. 肝胆胰外科杂志，2006, 18(5): 269-270, 273.

沈振斌，秦新裕. ABCG5和ABCG8在胆囊胆固醇结石形成中的作用[J]. 生命的化学，2004, 24(4): 340-341.

王勇，韩天权，张圣道. ABCG5和ABCG8与胆固醇代谢关系的研究进展[J]. 国际外科学杂志，2006, 33(2): 125-128.

蒋兆彦，韩天权，所广军，等. 胆固醇结石病人肝脏胆小管侧膜ATP基因表达差异的研究[J]. 外科理论与实践，2005, 10(1): 61-65.

王勇，吴卫泽，韩天权，等. ABCG8基因T400K多态性与胆囊结石病以及血脂水平的关系[J]. 外科理论与实践，2006, 11(6): 510-513.

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