消囊促卵丸对多囊卵巢综合征模型大鼠性激素水平的影响及相关作用机制研究
Effect of Xiaonang Culuan Wan on sex hormone levels of polycystic ovary syndrome model rats and its mechanism
- 上海中医药杂志 2021年55卷第11期 页码：74-79
- 作者机构：
1.湖南中医药大学第二附属医院药剂科（湖南 长沙 410000）
2.湖南中医药大学第二附属医院妇科（湖南 长沙 410000）
- 作者简介：
崔姣，女，硕士研究生，主管中药师，主要从事中药药理学与临床中药学方面研究工作
邹菊英，副主任药师；E-mail：418328009@qq.com
- 基金信息：湖南省中医药科研计划项目(202012)
DOI：10.16305/j.1007-1334.2021.2102015
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崔姣, 匡继林, 孙劲松, 等. 消囊促卵丸对多囊卵巢综合征模型大鼠性激素水平的影响及相关作用机制研究[J]. 上海中医药杂志, 2021,55(11):74-79.
Jiao CUI, Jilin KUANG, Jinsong SUN, et al. Effect of Xiaonang Culuan Wan on sex hormone levels of polycystic ovary syndrome model rats and its mechanism[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(11):74-79.
崔姣, 匡继林, 孙劲松, 等. 消囊促卵丸对多囊卵巢综合征模型大鼠性激素水平的影响及相关作用机制研究[J]. 上海中医药杂志, 2021,55(11):74-79. DOI： 10.16305/j.1007-1334.2021.2102015.
Jiao CUI, Jilin KUANG, Jinsong SUN, et al. Effect of Xiaonang Culuan Wan on sex hormone levels of polycystic ovary syndrome model rats and its mechanism[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(11):74-79. DOI： 10.16305/j.1007-1334.2021.2102015.
摘要
目的,2,探究消囊促卵丸对多囊卵巢综合征模型大鼠性激素水平的影响及相关作用机制。,方法,2,90只6周龄SPF级SD雌性大鼠，随机选取15只作为正常组（灌胃0.9%NaCl溶液）。剩余大鼠建立多囊卵巢综合征模型，建模成功后随机分为模型组（灌胃0.9%NaCl溶液）、阳性药物组（灌胃270 mg/kg二甲双胍）、低剂量组（灌胃3.15 g/kg消囊促卵丸）、中剂量组（灌胃6.30 g/kg消囊促卵丸）、高剂量组（12.60 g/kg消囊促卵丸），每组15只。造模成功后分别给予各组药物干预12 d。观察各组大鼠病理组织学变化，检测空腹胰岛素（FINS）、空腹血糖（FPG）、餐后2 h胰岛素（2 h INS）、餐后2 h血糖（2 h PG）、C-反应蛋白（CRP）、白介素-6（IL-6）、肿瘤坏死因子-α（TNF-α）、睾酮（T）、雌二醇（E,2,）、黄体生成素（LH）、促卵泡激素（FSH）水平及卵巢组织中,AKT,-2、,GSK,-3β、,IRS,-1、,GLUT,-4、,PTEN, mRNA表达量。分析消囊促卵丸对多囊卵巢综合征大鼠性激素水平的影响机制。,结果,2,与模型组比较，高剂量组、中剂量组、低剂量组、阳性药物组腹腔葡萄糖耐量（IPGTT）、炎性因子、T、E,2,、LH水平，,PTEN, mRNA表达量降低（,P,<,0.05）；与模型组比较，高剂量组、中剂量组、低剂量组、阳性药物组FSH水平，,AKT,-2、,GSK,-3β、,IRS,-1、,GLUT,-4 mRNA表达量升高（,P,<,0.05）；以高剂量组效果最佳。,结论,2,消囊促卵丸可能通过作用于PI3K/AKT信号通路，上调,AKT,-2、,GSK,-3β、,IRS,-1、,GLUT,-4 mRNA表达量，下调,PTEN, mRNA表达量，促进性功能，减轻炎症反应。
Abstract
Objective,2,To explore the effect of Xiaonang Culuan Wan (XCW) on the levels of sex hormones in rats with polycystic ovary syndrome and its related mechanism of action.,Methods,2,Of 90 six-week-old SPF female SD rats, 15 were randomly selected as normal group (intragastric administratiion of 0.9% NaCl solution). After successful establishment of polycystic ovary syndrome model, the remaining rats were randomly divided into model group (intragastric administratiion of 0.9%NaCl solution), positive drug group (intragastric administratiion of 270 mg/kg metformin), low-dose group (intragastric administratiion of 3.15 g/kg XCW), medium-dose group (intragastric administratiion of 6.30 g/kg XCW) and high-dose group (intragastric administratiion of 12.60 g/kg XCW), with 15 rats in each group. After successful modeling, each group was given drug intervention for 12 days. The histopathological changes of rats in each group were observed, and the levels of fasting insulin (FINS), fasting plasma glucose (FPG), 2-h postprandial insulin (2 h INS), 2-h postprandial glucose (2 h PG), C-reactive protein (CRP), interleukin 6 (IL-6), tumor necrosis factor -α (TNF-α) 、testosterone (T), estradiol (E,2,), luteinizing hormone (LH), follicle stimulating hormone (FSH) and the expression of ,AKT,-2,GSK,-3β、 ,IRS,-1,GLUT,-4,PTEN, mRNA in ovarian tissue were detected, which were used to analyze the mechanism and the effect of XCW on sex hormone levels in rats with polycystic ovary syndrome.,Results,2,Compared with the model group, the results of intraperitoneal glucose tolerance test (IPGTT) and levels of inflammatory factors, T, E,2, LH and ,PTEN, mRNA expression in the high-dose group, medium-dose group, low-dose group and positive drug group showed lower (,P,<,0.05); Compared with the model group, the level of FSH and levels of ,AKT,-2,GSK,-3β,IRS,-1 and ,GLUT,-4 mRNA expression in the high-dose, medium-dose, low-dose and positive drug groups were higher (,P,<,0.05), and the effect of high-dose group were the best.,Conclusion,2,By acting on P13K/AKT signal pathway, XCW can promote sexual function and reduce inflammation maybe through up-regulating the expression of ,AKT,-2,GSK,-3β,IRS,-1,GLUT,-4 mRNA and down-regulating the expression of ,PTEN, mRNA.
关键词
消囊促卵丸多囊卵巢综合征性激素作用机制模型大鼠中药研究
Keywords
Xiaonang Culuan Wanpolycystic ovary syndromesex hormonesmechanism of actionmodel ratstraditional Chinese herbal medicine research
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