茯苓丸加减方对兔膝骨关节炎模型MMP-3、MMP-13及TIMP-1表达水平的影响

安帅; 白荣闯; 李格格; 朱龙; 杨英昕

doi:10.16305/j.1007-1334.2021.2101075

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茯苓丸加减方对兔膝骨关节炎模型MMP-3、MMP-13及TIMP-1表达水平的影响

实验研究 | 更新时间：2022-08-03

- 茯苓丸加减方对兔膝骨关节炎模型MMP-3、MMP-13及TIMP-1表达水平的影响
- Effect of Modified Fulingwan Decoction on expression of MMP-3, MMP-13 and TIMP-1 in rabbit knee osteoarthritis model
- 上海中医药杂志 2021年55卷第7期页码：64-67
- 作者机构：
  
  1.辽宁中医药大学（辽宁　沈阳　110847）
  2.沈阳维康医院（辽宁　沈阳　110021）
  3.中国人民解放军北部战区总医院（辽宁　沈阳　110034）
- 作者简介：
  
  安帅，男，硕士，医师，主要从事软组织损伤基础与临床研究工作
  杨英昕，主任医师；E-mail：qjrszx@163.com
- 基金信息：
  
  中国人民解放军后勤保障部区域性军事训练伤监测与干预建设专项课题;军委后勤保障部卫生局-区域性军事训练伤监测干预中心试点建设项目(CSY12J004)
- DOI：10.16305/j.1007-1334.2021.2101075
  中图分类号：
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安帅, 白荣闯, 李格格, 等. 茯苓丸加减方对兔膝骨关节炎模型MMP-3、MMP-13及TIMP-1表达水平的影响[J]. 上海中医药杂志, 2021,55(7):64-67.

Shuai AN, Rongchuang BAI, Gege LI, et al. Effect of Modified Fulingwan Decoction on expression of MMP-3, MMP-13 and TIMP-1 in rabbit knee osteoarthritis model[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(7):64-67.
安帅, 白荣闯, 李格格, 等. 茯苓丸加减方对兔膝骨关节炎模型MMP-3、MMP-13及TIMP-1表达水平的影响[J]. 上海中医药杂志, 2021,55(7):64-67. DOI： 10.16305/j.1007-1334.2021.2101075.

Shuai AN, Rongchuang BAI, Gege LI, et al. Effect of Modified Fulingwan Decoction on expression of MMP-3, MMP-13 and TIMP-1 in rabbit knee osteoarthritis model[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(7):64-67. DOI： 10.16305/j.1007-1334.2021.2101075.

摘要

目的,2,观察茯苓丸加减方对兔膝骨关节炎（KOA）模型MMP-3、MMP-13及TIMP-1表达的影响。,方法,2,将日本大耳白兔随机分为正常组、模型组、双氯芬酸钠组和茯苓丸加减方组，每组6只；除正常组外，各组均采用改良Hulth法对其左后膝关节进行手术造模；造模成功后对各组大耳白兔进行4周的干预治疗，治疗结束后进行病理观察，同时采用ELISA 法对各组大耳白兔膝关节液内MMP-3、MMP-13和TIMP-1水平进行测定。,结果,2,模型组MMP-3、MMP-13和TIMP-1含量较正常组均明显升高（,P,<,0.01）；与模型组比较，双氯芬酸钠组和茯苓丸加减方组均明显下降（,P,<,0.01）。,结论,2,MMP-3、MMP-13和TIMP-1与KOA发病关系密切，茯苓丸加减方可能是通过抑制其病理性升高对KOA的发病起到治疗作用。

Abstract

Objective,2,To observe the effect of Modified Fulingwan Decoction on the expression of MMP-3, MMP-13 and TIMP-1 in rabbit knee osteoarthritis model.,Methods,2,Twenty-four Japan big ear white rabbits were randomly divided into normal group, model group, diclofenac sodium group and Modified Fulingwan Decoction group, 6 rabbits in each group. Except for the normal group, the modified Hulth method was used on the left posterior knee joint in other groups. After successful modeling, each group was intervened for four weeks. After the treatment cycle, and pathological observation was performed after the treatment. At the same time, ELISA was used to detect the levels of MMP-3, MMP-13 and TIMP-1 in the knee joint fluid of each groups’ subjects.,Results,2,The contents of MMP-3, MMP-13 and TIMP-1 in model group were significantly increased than those in the normal group (,P,<,0.01) . The contents in diclofenac sodium group and modified Fulingwan group were significantly decreased than those in model group (,P,<,0.01) .,Conclusions,2,MMP-3, MMP-13 and TIMP-1 are closely related to the pathogenesis of KOA. Modified Fuling Decoction may have a therapeutic effect on the pathogenesis of KOA by inhibiting their pathological increase.

关键词

茯苓丸加减方膝骨性关节炎MMP-3MMP-13TIMP-1

Keywords

Modified Fulingwan Decoctionknee osteoarthritisMMP-3MMP-13TIMP-1

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