丹参饮预处理对心肌缺血/再灌注大鼠心肌损伤和心功能影响的研究
Effect of Danshen Decoction preconditioning on myocardial injury and cardiac function in rats with myocardial ischemia/reperfusion
- 上海中医药杂志 2021年55卷第6期 页码：61-66
- 作者机构：
1.上海中医药大学附属曙光医院心内科（上海 201203）
2.上海中医药大学附属曙光医院心血管病研究所（上海 201203）
3.上海中医药大学附属曙光医院病理科（上海 201203）
- 作者简介：
呼明哲，女，硕士研究生，主要从事中西医结合心血管疾病研究工作
高俊杰，副主任医师，硕士研究生导师；E-mail：junjiegao@live.cn
- 基金信息：国家自然科学基金项目(81703743)
DOI：10.16305/j.1007-1334.2021.2101068
中图分类号：
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呼明哲, 李湘玲, 杨涛, 等. 丹参饮预处理对心肌缺血/再灌注大鼠心肌损伤和心功能影响的研究[J]. 上海中医药杂志, 2021,55(6):61-66.
Mingzhe HU, Xiangling LI, Tao YANG, et al. Effect of Danshen Decoction preconditioning on myocardial injury and cardiac function in rats with myocardial ischemia/reperfusion[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(6):61-66.
呼明哲, 李湘玲, 杨涛, 等. 丹参饮预处理对心肌缺血/再灌注大鼠心肌损伤和心功能影响的研究[J]. 上海中医药杂志, 2021,55(6):61-66. DOI： 10.16305/j.1007-1334.2021.2101068.
Mingzhe HU, Xiangling LI, Tao YANG, et al. Effect of Danshen Decoction preconditioning on myocardial injury and cardiac function in rats with myocardial ischemia/reperfusion[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(6):61-66. DOI： 10.16305/j.1007-1334.2021.2101068.
摘要
目的,2,观察丹参饮预处理对心肌缺血/再灌注损伤（MI/RI）大鼠心肌梗死面积、心功能和炎症因子的影响。,方法,2,超高效液相色谱-高分辨质谱联用分析法分析丹参饮的化学成分；将Wistar大鼠随机分为假手术组、模型组、丹参饮低剂量预处理I/R组、丹参饮中剂量预处理I/R组、丹参饮高剂量预处理I/R组；给药1周后，采用大鼠冠状动脉前降支结扎30 min/复流24 h的方法建立心肌缺血/再灌注模型；2,3,5-氯化三苯基四氮唑染色观察心肌梗死面积，ImageJ软件计算心肌梗死面积；使用心脏彩色多普勒仪观察各组大鼠左室射血分数（LVEF）和左室缩短率（LVFS）；采用ELISA法测定各组大鼠血清白介素-6（IL-6）和肿瘤坏死因子-α（TNF-α）的表达。,结果,2,①丹参饮的主要化学成分为丹酚酸B、丹参酮ⅡA、槲皮素、槲皮苷。②与模型组比较，丹参饮各剂量组大鼠心肌梗死面积均有下降（,P,<,0.05）。③与假手术组比较，模型组大鼠的LVEF和LVFS均明显降低（,P,<,0.01）；与模型组比较，丹参饮各剂量预处理I/R组大鼠的LVEF和LVFS均有不同程度改善（,P,<,0.05，,P,<,0.01）。④与假手术组比较，模型组大鼠血清中IL-6和TNF-α含量均有增加（,P,<,0.01）；与模型组比较，丹参饮各剂量预处理I/R组大鼠血清IL-6水平均明显下降（,P,<,0.01），丹参饮低、中剂量预处理I/R组TNF-α水平亦均有下降（,P,<,0.05）。,结论,2,丹参饮预处理具有减少MI/RI大鼠心肌梗死面积、改善心功能的作用，其机制可能与减少缺血再灌注时血清炎症因子的表达相关。
Abstract
Objective,2,To observe the effect of Danshen Decoction (DSY) pretreatment on infarct size, cardiac function and inflammatory cytokines in serum in rats with myocardial ischemia/reperfusion injury (MI/RI).,Methods,2,Chemical components of DSY were analyzed by ultra-high performance liquid chromatography/ultra-resolution mass spectrometry. Thirty Wistar rats were randomly divided into five groups: sham operation group, ischemia/reperfusion (I/R) model group, DSY (low-dose, medium-dose and high-dose) pretreatment I/R groups, with 6 rats in each group. After 1 week of continuous gavage, the rat model of myocardial ischemia/reperfusion (I/R) was established by ligation of the anterior descending coronary artery for 30 min/reflow for 24h. TTC staining was used to observe the infarct size, which was measured by ImageJ software. Left ventricular ejection fraction (LVEF) and left ventricular fraction shortening (LVFS) were observed by Cardiac Color Ultrasound Doppler. The levels of interleukin-6 (IL-6) and tumor necrosis factor-α (TNF-α) in serum of each rat group were determined by ELISA.,Results,2,①The main chemical components of DSY were salvianolic acid B, tanshinone IIA, quercetin and quercitrin. ②Compared with the model group, the size of myocardial infarction was decreased in DSY (low-dose, medium-dose and high dose) preconditioning groups (,P,<,0.05). ③Compared with sham operation group, LVEF and LVFS in I/R model group were significantly decreased (,P,<,0.05,P,<,0.01). Compared with I/R model group, the LVEF and LVFS of rats in DSY (low-dose, medium-dose and high dose) preconditioning groups were improved (,P,<,0.05). ④Compared with the sham operation group, the levels of IL-6 and TNF-α in serum in I/R model group were increased (,P,<,0.01). Compared with I/R model group, the levels of IL-6 in serum in DSY (low-dose, medium-dose and high dose) pretreatment groups were decreased (,P,<,0.01). The levels of TNF-α in serum in DSY (low-dose and medium-dose) pretreatment groups were also decreased (,P,<,0.05). There was no significant difference of TNF-α expression between the high-dose group and the model group (,P,>,0.05).,Conclusion,2,DSY pretreatment has the effect of reducing infarct size, improving cardiac function of MI/RI rats, whose mechanism may be related to the reduction of the expression of inflammatory cytokines in serum of MI/RI rats.
关键词
丹参饮心肌缺血/再灌注损伤心功能炎症因子心肌保护
Keywords
Danshen Decoctionmyocardial ischemia/reperfusion injurycardiac functioninflammatory cytokinescardioprotection
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