黄芪甲苷基于MEK5/ERK5信号通路对阿尔茨海默病大鼠小胶质细胞活性的影响
Effect of astragaloside Ⅳ on microglia activity in Alzheimer’s disease rats based on MEK5/ERK5 signaling pathway
- 上海中医药杂志 2021年55卷第10期 页码：73-78
- 作者机构：
1.河南大学第一附属医院神经内科（河南 开封 475001）
- 作者简介：
方建，男，硕士，副主任医师，主要从事认知功能障碍和痴呆的临床和基础研究工作
陈文武，主任医师；E-mail：sxto53@163.com
- 基金信息：河南省医学科技攻关项目(2017T02048);开封市科技发展计划项目(1908008)
DOI：10.16305/j.1007-1334.2021.2101040
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方建, 李晓晖, 陈文武. 黄芪甲苷基于MEK5/ERK5信号通路对阿尔茨海默病大鼠小胶质细胞活性的影响[J]. 上海中医药杂志, 2021,55(10):73-78.
Jian FANG, Xiaohui LI, Wenwu CHEN. Effect of astragaloside Ⅳ on microglia activity in Alzheimer’s disease rats based on MEK5/ERK5 signaling pathway[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(10):73-78.
方建, 李晓晖, 陈文武. 黄芪甲苷基于MEK5/ERK5信号通路对阿尔茨海默病大鼠小胶质细胞活性的影响[J]. 上海中医药杂志, 2021,55(10):73-78. DOI： 10.16305/j.1007-1334.2021.2101040.
Jian FANG, Xiaohui LI, Wenwu CHEN. Effect of astragaloside Ⅳ on microglia activity in Alzheimer’s disease rats based on MEK5/ERK5 signaling pathway[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(10):73-78. DOI： 10.16305/j.1007-1334.2021.2101040.
摘要
目的,2,研究黄芪甲苷基于丝裂原激活蛋白激酶5（MEK5）/细胞外信号调节激酶5（ERK5）信号通路对阿尔茨海默病（AD）大鼠小胶质细胞活性的影响。,方法,2,清洁级健康SD雄性大鼠60只，随机选取10只为正常组，其余50只建立AD模型，然后随机分为模型组、阳性对照组、黄芪甲苷低剂量组、黄芪甲苷中剂量组、黄芪甲苷高剂量组，每组10只。阳性对照组给予石杉碱甲（0.02 mg/kg）干预，正常组和模型组分别给予等体积的0.9%NaCl溶液，黄芪甲苷低剂量组、黄芪甲苷中剂量组、黄芪甲苷高剂量组分别给予15 mg/kg、30 mg/kg、60 mg/kg的黄芪甲苷。各组均干预21 d，检测白介素-1（IL-1）、肿瘤坏死因子-α（TNF-α）水平，以及葡萄糖调节蛋白78（GRP78）、C/EBP同源蛋白（CHOP）、MEK5、ERK5表达。,结果,2,与正常组相比，模型组、阳性对照组、黄芪甲苷低剂量组、黄芪甲苷中剂量组、黄芪甲苷高剂量组IL-1、TNF-α水平及GRP78、CHOP、MEK5、ERK5表达升高（,P,<,0.05）；与模型组相比，阳性对照组、黄芪甲苷低剂量组、黄芪甲苷中剂量组、黄芪甲苷高剂量组IL-1、TNF-α水平及GRP78、CHOP、MEK5、ERK5表达降低，且随剂量增加而降低（,P,<,0.05）。,结论,2,黄芪甲苷基于MEK5/ERK5信号通路对AD大鼠小胶质细胞的活性具有抑制作用，可减少AD大鼠神经细胞的凋亡。
Abstract
Objective,2,To investigate the effect of Astragaloside Ⅳ on microglia activity in Alzheimer’s disease (AD) rats based on mitogen activated protein kinase 5 (MEK5)/extracellular signal regulated kinase 5 (ERK5) signaling pathway.,Methods,2,Sixty clean grade healthy SD male rats were selected, and 10 were randomly selected as a normal group. The remaining 50 were randomly divided into the model group, the positive control group, the astragaloside Ⅳ low dose group, the Astragaloside Ⅳ medium dose group and the Astragaloside Ⅳ high dose group after the establishment of the AD model, with 10 rats in each group. The positive control group was intervened with huperzine A (0.02 mg/kg), the normal group and model group were administered with the equal volume of 0.9% NaCl solution, and the astragaloside Ⅳ low dose group, the Astragaloside Ⅳ medium dose group and the astragaloside Ⅳ high dose group were administered with 15 mg/kg, 30 mg/kg and 60 mg/kg of Astragaloside Ⅳ, respectively. All groups were intervened for 21 days. Interleukin-1 (IL-1) and tumor necrosis factor-α (TNF-α) levels as well as the expressions of glucose-regulated protein 78 (GRP78), C/EBP homologous protein (CHOP), MEK5 and ERK5 were tested.,Results,2,Compared with normal group, the levels of IL-1, TNF-α and the expressions of GRP78, CHOP, MEK5 and ERK5 in model group, positive control group, low dose astragaloside Ⅳ group, medium dose astragaloside Ⅳ group and high dose astragaloside Ⅳ group were increased (,P,<,0.05). Compared with model group, the levels of IL-1, TNF-α levels and the expressions of GRP78, CHOP, MEK5 and ERK5 in positive control group, low dose astragaloside Ⅳ group, medium dose astragaloside Ⅳ group and high dose astragaloside Ⅳ group were decreased in a dose-dependent manner, and also decreased with dose increase (,P,<,0.05).,Conclusion,2,Astragaloside Ⅳ can inhibit the activity of microglia and reduce the apoptosis of neurons in AD rats based on MEK5 /ERK5 signaling pathway.
关键词
黄芪甲苷丝裂原激活蛋白激酶5细胞外信号调节激酶5阿尔茨海默病小胶质细胞模型大鼠中药研究
Keywords
astragaloside Ⅳmitogen activated protein kinase 5extracellular signal regulated kinase 5Alzheimer’s diseasemicrogliamodel ratresearch of traditional Chinese herbal medicine
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