不同浓度艾烟对大鼠肺组织影响的比较研究

王丽莉; 陈侃俊; 余安胜; 吴欢; 窦丹波

doi:10.16305/j.1007-1334.2021.2012089

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不同浓度艾烟对大鼠肺组织影响的比较研究

实验研究 | 更新时间：2022-08-03

- 不同浓度艾烟对大鼠肺组织影响的比较研究
- Comparative study on the effect of different concentrations of moxa smoke on lung tissue of rats
- 上海中医药杂志 2021年55卷第6期页码：79-83
- 作者机构：
  
  1.上海中医药大学附属曙光医院传统中医科（上海　201203）
- 作者简介：
  
  王丽莉，女，硕士，副主任医师，主要从事中医综合疗法治疗内科疾病的临床研究工作
  窦丹波，主任医师，硕士研究生导师；E-mail：doudanbo@shutcm.edu.cn
- 基金信息：
  
  国家自然科学基金青年科学基金项目(81704150)
- DOI：10.16305/j.1007-1334.2021.2012089
  中图分类号：
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王丽莉, 陈侃俊, 余安胜, 等. 不同浓度艾烟对大鼠肺组织影响的比较研究[J]. 上海中医药杂志, 2021,55(6):79-83.

Lili WANG, Kanjun CHEN, Ansheng YU, et al. Comparative study on the effect of different concentrations of moxa smoke on lung tissue of rats[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(6):79-83.
王丽莉, 陈侃俊, 余安胜, 等. 不同浓度艾烟对大鼠肺组织影响的比较研究[J]. 上海中医药杂志, 2021,55(6):79-83. DOI： 10.16305/j.1007-1334.2021.2012089.

Lili WANG, Kanjun CHEN, Ansheng YU, et al. Comparative study on the effect of different concentrations of moxa smoke on lung tissue of rats[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(6):79-83. DOI： 10.16305/j.1007-1334.2021.2012089.

摘要

目的,2,探究不同浓度艾烟对大鼠肺组织的毒性作用及其潜在的分子机制。,方法,2,将20只SD大鼠随机分为对照组、低浓度艾烟组、中浓度艾烟组、高浓度艾烟组；艾烟组大鼠分别放置于低、中、高浓度艾烟的染毒柜中，每天1 h，持续8周。比较各组大鼠肺灌洗液中SOD、TNF-α、IL-6和IL-10的表达情况；检测肺组织病理变化及NF-κB、TLR2和TLR4蛋白的表达情况。,结果,2,①中低浓度艾烟组大鼠体质量显著高于对照组，而高浓度艾烟组大鼠体质量显著低于对照组（,P,<,0.001）。②中高浓度艾烟组大鼠肺组织有明显的炎症细胞浸润，高浓度艾烟组大鼠肺组织发生纤维化；③中高浓度艾烟组大鼠肺组织中IL-6、TNF-α、NF-κB、TLR2和TLR4等炎症因子的表达显著增加， SOD和IL-10的表达降低（,P,<,0.05）。,结论,2,低浓度艾烟对机体无显著性损害，高浓度艾烟引起的肺部损伤主要是通过促进炎症因子的表达产生的。

Abstract

Objective,2,To investigate the toxic effects of different concentrations of moxa smoke on rat lung tissues and to explore the potential molecular mechanisms.,Methods,2,Twenty SD rats were randomly divided into four groups: control group, low concentration group of moxa smoke, medium concentration group of moxa smoke and high concentration group of moxa smoke. Rats in the moxa smoke groups were respectively placed in the poisoning cabinet of low, medium and high concentration of moxa smoke for 1 hour per day for 8 weeks. The expressions of SOD, TNF-α, IL-6 and IL-10 in the lung lavage fluid of different groups were compared. The pathological changes of lung tissue and the expressions of NF-κB, TLR2 and TLR4 proteins were detected.,Results,2,①The body weight of rats in the medium and low concentration groups of moxa smoke was significantly higher than that of the control group, while the body weight of rats in the high concentration group of moxa smoke was significantly lower than that of the control group (,P,<,0.001) . ②There were significantly inflammatory cell infiltration in the lung tissue of the medium and high concentration groups, and fibrosis occurred in the lung tissue of rats in the high concentration group. ③The expressions of inflammatory factors such as IL-6, TNF-α, NF-κB, TLR2 and TLR4 in the rat lung tissue of the medium and high concentrations groups of moxa smoke were significantly increased, while the expressions of SOD and IL-10 were decreased (,P,<,0.05) .,Conclusion,2,Low concentrations of moxa smoke do not significantly damage the body, and the lung damage induced by high concentrations of moxa smoke is mainly produced by promoting the expression of inflammatory factors.

关键词

艾烟模型大鼠肺部损伤炎症因子

Keywords

moxa smokemodel ratslung injuryinflammatory factors

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