平喘方对哮喘急性发作期小鼠MMP-9、TIMP-1、E-cadherin及CollagenⅠ的影响

杨艳; 薛征; 虞坚尔; 胡逸中; 刘亚尊; 徐万超

doi:10.16305/j.1007-1334.2021.2009038

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平喘方对哮喘急性发作期小鼠MMP-9、TIMP-1、E-cadherin及CollagenⅠ的影响

实验研究 | 更新时间：2022-08-03

- 平喘方对哮喘急性发作期小鼠MMP-9、TIMP-1、E-cadherin及CollagenⅠ的影响
- Effect of Pingchuan Formula on MMP-9, TIMP-1, E-cadherin and CollagenⅠ in asthmatic mice at acute stage
- 上海中医药杂志 2021年55卷第6期页码：67-71
- 作者机构：
  
  1.上海中医药大学附属市中医医院儿科（上海　200071）
  2.上海中医药大学研究生院（上海　201203）
- 作者简介：
  
  杨艳，女，硕士研究生，主要从事小儿肺系疾病研究工作
  薛征，主任医师，教授，博士研究生导师；E-mail：xz695@163.com
- 基金信息：
  
  国家自然科学基金项目(81874488)
- DOI：10.16305/j.1007-1334.2021.2009038
  中图分类号：
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杨艳, 薛征, 虞坚尔, 等. 平喘方对哮喘急性发作期小鼠MMP-9、TIMP-1、E-cadherin及CollagenⅠ的影响[J]. 上海中医药杂志, 2021,55(6):67-71.

Yan YANG, Zheng XUE, Jianer YU, et al. Effect of Pingchuan Formula on MMP-9, TIMP-1, E-cadherin and CollagenⅠ in asthmatic mice at acute stage[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(6):67-71.
杨艳, 薛征, 虞坚尔, 等. 平喘方对哮喘急性发作期小鼠MMP-9、TIMP-1、E-cadherin及CollagenⅠ的影响[J]. 上海中医药杂志, 2021,55(6):67-71. DOI： 10.16305/j.1007-1334.2021.2009038.

Yan YANG, Zheng XUE, Jianer YU, et al. Effect of Pingchuan Formula on MMP-9, TIMP-1, E-cadherin and CollagenⅠ in asthmatic mice at acute stage[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(6):67-71. DOI： 10.16305/j.1007-1334.2021.2009038.

摘要

目的,2,通过检测小鼠肺组织中基质金属蛋白酶9（MMP-9）、金属蛋白酶组织抑制因子1（TIMP-1）、E-钙黏蛋白（E-cadherin）、Ⅰ型胶原（CollagenⅠ）的蛋白表达水平，探讨平喘方对哮喘急性发作期小鼠气道重塑的作用机制。,方法,2,将40只Balb/c雄性小鼠按随机数字表分为空白组、模型组、地塞米松组、平喘方组，每组10只。除空白组外，其余组均采用OVA“致敏＋激发”建立哮喘模型。灌胃给药干预1周后，采用Masson染色观察肺组织病理变化，ELISA法检测血小板衍生生长因子（PDGF）水平，Western blot法检测MMP-9、TIMP-1、E-cadherin、CollagenⅠ蛋白含量。,结果,2,空白组可见少许淡蓝色胶原纤维，胶原容积分数（CVF）最小，与空白组比较，模型组胶原纤维面积明显增多（,P,<,0.01），颜色更深；与模型组比较，地塞米松组、平喘方组小鼠胶原纤维面积明显较少（,P,<,0.01）。与空白组比较，模型组小鼠PDGF因子水平、MMP-9、TIMP-1、CollagenⅠ蛋白表达水平显著上升（,P,<,0.01），E-cadherin蛋白表达水平显著降低（,P,<,0.01）；与模型组比较，地塞米松组及平喘方组小鼠PDGF因子水平、TIMP-1、CollagenⅠ均显著下降（,P,<,0.01，,P,<,0.05），E-cadherin蛋白表达水平均显著上升（,P,<,0.01），地塞米松组MMP-9蛋白表达水平显著升高（,P,<,0.01），平喘方组MMP-9蛋白水平升高，但不显著（,P,>,0.05）。,结论,2,在哮喘急性发作期，平喘方可通过抑制PDGF的释放，降低MMP-9、TIMP-1、CollagenⅠ的表达以及上调E-cadherin的表达来缓解气道重塑，从而发挥哮喘治疗作用，为化痰药与祛瘀药相配治疗哮喘提供了理论依据。

Abstract

Objective,2,To explore the mechanism of Pingchuan Formula on airway remodeling in mice with acute asthma attacks by detecting the expression levels of matrix metalloproteinase 9 (MMP-9), tissue inhibitor of matrix metalloprotease-1 (TIMP-1), E-cadherin and collagen type Ⅰ (Collagen Ⅰ) proteins in the lung tissue of mice.,Methods,2,According to the random number table, 40 Balb/c male mice were divided into blank group, model group, dexamethasone group and Pingchuan Formula group, with 10 mice in each group. The groups except the blank group were modeled by OVA "sensitization + stimulation" . After 1 week of gavage administration, the lung tissue pathology was observed by Masson staining. The level of platelet-derived growth factor (PDGF) was detected by ELISA method. The protein contents of MMP-9, TIMP-1, E-cadherin and Collagen Ⅰ was detected by Western blot method.,Results,2,Some light blue collagen fibers could be seen in the blank group, and the collagen volume fraction(CVF) was the smallest. Compared with the blank group, the area of collagen fibers in the model group was significantly increased (,P,<,0.01), and the color was darker; compared with the model group, the area of collagen fibers in the dexamethasone group and the Pingchuan Formula group was significantly decreased (,P,<,0.01). Compared with the blank group, the PDGF factor level and the MMP-9、TIMP-1、Collagen Ⅰ protein expression levels of mice in the model group increased significantly (,P,<,0.01), but the expression level of E-cadherin protein decreased significantly (,P,<,0.01); compared with the model group, the PDGF factor level and TIMP-1、Collagen Ⅰ protein expression levels of mice in dexamethasone group and Pingchuan Formula group decreased significantly (,P,<,0.01,P,<,0.05), and the E-cadherin protein expression level increased significantly (,P,<,0.01). The expression level of MMP-9 protein was significantly increased in dexamethasone group (,P,<,0.01), while that was increased but not significant in Pingchuan Formula group (,P,>,0.05).,Conclusion,2,Pingchuan Formula can relieve airway remodeling by inhibiting PDGF release, reducing MMP-9、TIMP-1、CollagenⅠ expression and up-regulating E-cadherin expression, which provides a theoretical evidence for the clinical use of phlegm-removing drugs combined with blood stasis -removing drugs in the treatment of asthma.

关键词

平喘方哮喘模型小鼠气道重塑基质金属蛋白酶9金属蛋白酶组织抑制因子1E-钙黏蛋白Ⅰ型胶原

Keywords

Pingchuan Formulaasthmamodel miceairway remodelingMMP-9TIMP-1E-cadherinCollagenⅠ

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