基于菌群16S测序技术探究益和方对小鼠脂质代谢和骨髓炎症因子以及肠道微生态的影响

宋寒冰; 李雪; 申意伟; 李佐; 范桢亮; 张天

doi:10.16305/j.1007-1334.2021.2005063

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基于菌群16S测序技术探究益和方对小鼠脂质代谢和骨髓炎症因子以及肠道微生态的影响

实验研究 | 更新时间：2022-08-03

- 基于菌群16S测序技术探究益和方对小鼠脂质代谢和骨髓炎症因子以及肠道微生态的影响
- Effect of Yihefang on lipid metabolism, bone marrow inflammatory factors and intestinal microecology in mice based on 16S rDNA sequencing technology of microbiome
- 上海中医药杂志 2021年55卷第5期页码：70-76
- 作者机构：
  
  1.黑龙江中医药大学临床医学院（黑龙江　哈尔滨　150040）
  2.黑龙江中医药大学附属第一医院骨伤科（黑龙江　哈尔滨　150040）
  3.黑龙江中医药大学附属第二医院骨伤科（黑龙江　哈尔滨　150001）
  4.东北师范大学生命科学院（吉林　长春　130024）
  5.浙江省中医院肾病科（浙江　杭州　310006）
  6.暨南大学医学部（广东　广州　510632）
- 作者简介：
  
  宋寒冰，男，博士，副主任医师，主要从事中西医结合防治骨伤科疾病的临床研究工作
  申意伟，博士；E-mail：yellwell@qq.com
- 基金信息：
  
  国家自然科学基金项目(81904222);黑龙江省普通本科高等学校青年创新人才培养计划项目(UNPYSCP-2017228);黑龙江省中医药科研项目(ZHY19-033);黑龙江中医药大学国家级配套项目(2019PT08);黑龙江中医药大学科研基金项目(201811);黑龙江中医药大学“优秀创新人才支持计划”科研项目(2018RCQ07);黑龙江省博士后项目(LBH-Z20034)
- DOI：10.16305/j.1007-1334.2021.2005063
  中图分类号：
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宋寒冰, 李雪, 申意伟, 等. 基于菌群16S测序技术探究益和方对小鼠脂质代谢和骨髓炎症因子以及肠道微生态的影响[J]. 上海中医药杂志, 2021,55(5):70-76.

Hanbing SONG, Xue LI, Yiwei SHEN, et al. Effect of Yihefang on lipid metabolism, bone marrow inflammatory factors and intestinal microecology in mice based on 16S rDNA sequencing technology of microbiome[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(5):70-76.
宋寒冰, 李雪, 申意伟, 等. 基于菌群16S测序技术探究益和方对小鼠脂质代谢和骨髓炎症因子以及肠道微生态的影响[J]. 上海中医药杂志, 2021,55(5):70-76. DOI： 10.16305/j.1007-1334.2021.2005063.

Hanbing SONG, Xue LI, Yiwei SHEN, et al. Effect of Yihefang on lipid metabolism, bone marrow inflammatory factors and intestinal microecology in mice based on 16S rDNA sequencing technology of microbiome[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(5):70-76. DOI： 10.16305/j.1007-1334.2021.2005063.

摘要

目的,2,通过菌群16S rDNA测序技术，探讨益和方在调节小鼠肠道菌群微生态结构、骨髓异常炎症反应及血清脂质代谢中的作用。,方法,2,将30只雄性C57BL/6J小鼠随机分为正常组、高脂组与益和方组。高脂组给予高脂饮食诱导复制脂质代谢紊乱模型，益和方组在高脂组基础上给予等量益和方中药液灌胃。干预12周时收集小鼠新鲜粪便、血清及骨髓液样品，检测血清胆固醇及三酰甘油水平，qPCR及Western blot法检测炎症因子白介素-6（IL-6）水平，粪便样品行16S测序检测菌群多样性。,结果,2,与高脂组比较，益和方组小鼠体质量、血清胆固醇及三酰甘油水平均显著降低（,P,<,0.05，,P,<,0.01），骨髓中IL-6转录及蛋白表达均显著降低（,P,<,0.05），肠道菌群微生态结构趋于正常化。,结论,2,益和方可调节高脂饮食诱导的机体脂质代谢紊乱，缓解骨髓异常炎症反应，同时对肠道微生态失衡的恢复重建亦有积极作用。

Abstract

Objective,2,To explore the role of Yihefang in regulating the microecological structure of gut microbiota, bone marrow abnormal inflammation and serum lipid metabolism in mice by 16S rDNA sequencing technology.,Methods,2,Thirty male C57BL/6J mice were randomly divided into normal group, high-fat group and Yihefang group. The high-fat group was given a high-fat diet to induce a lipid metabolism disorder model, and the Yihefang group was given the same amount of Yihefang liquid on the basis of the high-fat group. At 12 weeks of the treatment, samples of fresh feces, serum and bone marrow fluid were collected from mice to detect serum cholesterol and triglyceride levels. qPCR and Western blot were used to detect IL-6 levels of inflammatory factors, and 16S rDNA sequencing to detect bacterial diversity of stool samples.,Results,2,Compared with the high-fat group, the body weight, serum cholesterol and triglyceride levels of mice in Yihefang group were significantly reduced (,P,<,0.05,P,<,0.01); IL-6 transcription and protein expression in bone marrow were significantly reduced (,P,<,0.05); and the microecological structure of gut microbiota tended to be normalization.,Conclusion,2,Yihefang can regulate the disorder of lipid metabolism induced by high-fat diet, alleviate the abnormal inflammatory reaction of bone marrow, and also play a positive role in the recovery and reconstruction of intestinal microecological imbalance.

关键词

脂质代谢高脂肪饮食模型小鼠肠道菌群炎症16S测序中药研究

Keywords

lipid metabolismhigh fat dietmodel micegut microbiotainflammation16S rDNA sequencingtraditional Chinese medicine research

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