食道通结方对人食管癌裸鼠移植瘤微血管生成的影响
Effect of Shidao Tongjie Decoction on micro angiogenesis of human esophageal carcinoma xenograft in nude mice
- 上海中医药杂志 2021年55卷第1期 页码：83-87
- 作者机构：
1.上海市胸科医院，上海交通大学附属胸科医院中西医结合科（上海 200030）
2.上海交通大学医学院（上海 200025）
- 作者简介：
蔡霄月，女，博士，主治医师，主要从事中西医结合治疗胸部肿瘤的临床和实验研究工作
张铭，主任医师，硕士研究生导师；E-mail：gason2000@126.com
- 基金信息：上海市科委科研计划项目(16401933400);上海交通大学“交大之星”计划医工交叉研究基金项目(YG2019QNA52)
DOI：10.16305/j.1007-1334.2021.2004198
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蔡霄月, 张铭, 周亚宁, 等. 食道通结方对人食管癌裸鼠移植瘤微血管生成的影响[J]. 上海中医药杂志, 2021,55(1):83-87.
Xiaoyue CAI, Ming ZHANG, Yaning ZHOU, et al. Effect of Shidao Tongjie Decoction on micro angiogenesis of human esophageal carcinoma xenograft in nude mice[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(1):83-87.
蔡霄月, 张铭, 周亚宁, 等. 食道通结方对人食管癌裸鼠移植瘤微血管生成的影响[J]. 上海中医药杂志, 2021,55(1):83-87. DOI： 10.16305/j.1007-1334.2021.2004198.
Xiaoyue CAI, Ming ZHANG, Yaning ZHOU, et al. Effect of Shidao Tongjie Decoction on micro angiogenesis of human esophageal carcinoma xenograft in nude mice[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(1):83-87. DOI： 10.16305/j.1007-1334.2021.2004198.
摘要
目的,2,观察食道通结方对人食管癌裸鼠移植瘤微血管生成的影响。,方法,2,通过皮下接种人食管癌TE-1细胞株建立人食管癌裸鼠移植瘤模型。将40只荷瘤鼠随机分为模型组、顺铂组、中药组、中药+顺铂组。各组分别予0.9%NaCl溶液、中药食道通结方（18 g·kg,-1,·d,-1,，灌胃，28 d）、顺铂（1 mg·kg,-1,·d,-1,，腹腔注射，d1、d3、d5）、食道通结方（18 g·kg,-1,·d,-1,，灌胃，28 d）+顺铂（1 mg·kg,-1,·d,-1,，腹腔注射，d1、d3、d5）干预。观察各组裸鼠移植瘤的生长情况，免疫组化法检测肿瘤组织CD34的表达，计算肿瘤微血管密度（MVD），免疫组化法检测肿瘤组织血管内皮细胞生长因子A（VEGFA）的表达，采用Western blot法检测肿瘤组织血管内皮细胞生长因子A（VEGFA）、血管内皮细胞生长因子受体2（VEGFR2）、磷酸化血管内皮生长因子受体2（p-VEGFR2）蛋白的表达。,结果,2,与模型组比较，各给药组移植瘤的体积及瘤重均明显减小（,P,<,0.01），以中药+顺铂组的抑瘤作用最强（,P,<,0.05）。与模型组比较，中药组、顺铂组、中药+顺铂组肿瘤MVD及VEGFA、p-VEGFR2的表达均明显降低（,P,<,0.05），且中药+顺铂组下调MVD及VEGFA、p-VEGFR2蛋白表达的作用较顺铂组更为显著（,P,<,0.05）。,结论,2,食道通结方具有拮抗食管癌血管新生的作用，该作用与抑制VEGFA/VEGFR2通路有关。
Abstract
Objective,2,To observe the effect of Shidao Tongjie Decoction on micro angiogenesis of human esophageal cancer xenografts in nude mice., Methods,2,The xenograft model of human esophageal carcinoma was established by subcutaneous inoculation of human esophageal carcinoma cell line TE-1. Forty tumor bearing mice were randomly divided into 4 groups: model group, Chinese medicine group, cisplatin group and Chinese medicine + cisplatin group. The mice in each group were intervened with 0.9%NaCl, Shidao Tongjie Decoction (18 g·kg,-1,·d,-1, ig, 28 d) , cisplatin (1 mg·kg,-1,·d,-1, ip, d1, d3, d5) , Shidao Tongjie Decoction (18 g·kg,-1,·d,-1, ig, 28 d) + cisplatin (1 mg·kg,-1,·d,-1, ip, d1, d3, d5) , respectively. The growth of transplanted tumor was observed. The expression of CD34 was detected by immunohistochemistry, and microvas cular density (MVD) was calculated according to the expression of CD34. The expressions of vascular endothelial growth factor A (VEGFA) protein was detected by immunohistochemistry.The expressions of VEGFA, vascular endothelial growth factor receptor 2 (VEGFR2) and p-VEGFR2 protein were detected by Western blot.,Results,2,Compared with the model group, the volume and weight of transplanted tumor in treatment groups were significantly reduced (,P,<,0.01) , and the effect of Chinese medicine plus cisplatin was the best (,P,<,0.05) . Compared with the model group, the expressions of MVD, VEGFA and p-VEGFR2 in the tumor tissues of the Chinese medicine group, cisplatin group and the Chinese medicine + cisplatin group decreased significantly (,P,<,0.05) , and the effects of the Chinese medicine + cisplatin group on the expression of MVD, VEGFA and p-VEGFR2 were more significant (,P,<,0.05) .,Conclusion,2,Shidao Tongjie Decoction has the effect of anti-angiogenesis of esophageal cancer, which is related to the inhibition of VEGFA/VEGFR2 pathway.
关键词
食道通结方食管癌小鼠肿瘤血管新生VEGFA/VEGFR2通路
Keywords
Shidao Tongjie Decoctionesophageal cancermicetumor angiogenesisVEGFA/VEGFR2 pathway
references
GRAHAM A J，SHRIVE F M，GHALI W A，et al. Defining the optimal treatment of locally advanced esophageal cancer：a systematic review and decision analysis[J].Ann Thorac Surg，2007,83(4):1257-1264.
查永嫱，孙东东，潘立群.食管癌术后中医药治疗研究进展[J].南京中医药大学学报，2019,35(6):767-770.
蔡霄月，张铭，金长娟，等.食道通结颗粒联合化疗对Ⅱb-Ⅲc期食管鳞癌根治术后患者无病生存期的影响[J].上海中医药杂志，2019,53(6):54-57.
蔡霄月，杨茜文，卢文峰，等.食道通结颗粒联合化疗治疗中晚期食管癌的近期疗效观察[J].上海中医药杂志，2017,51(7):44-47.
杨茜雯，张铭，金长娟，等.食道通结方辅助化疗对中晚期食管癌鳞癌患者生存期及免疫功能的影响[J].中医杂志，2017,58(21):1838-1841.
崔清，董昀，张铭，等.食道通结方对裸鼠食管癌移植瘤中TE-1细胞增殖及凋亡的影响[J].上海中医药杂志，2016,50(5):65-69.
董昀，张铭，金长娟，等.食道通结方对食管癌裸鼠移植瘤细胞增殖及凋亡的影响[J].上海中医药大学学报，2018,32(4):70-74.
张铭，李蕾，崔清，等.食道通结方对食管癌细胞株TE-1增殖和凋亡的影响[J].上海中医药杂志，2014,48(6):92-95.
WEIDNER N.Current pathologic methods for measuring intratumoral microvessel density within breast carcinoma and other solid tumors[J].Breast Cancer Res Treat，1995,36(2):169-180.
XIE B，WANG Y，HE J，et al.Aberrantcyclin E and hepatocyte growth factor expression，microvascular density and micro-lymphatic vessel density in esophageal squamous cell carcinoma[J].Cancer Control，2019,26(1):1073274819875736.
FERRARA N.Vascular endothelial growth factor：molecular and biological aspects[J].Curr Top Microbiol Immunol，1999(237):1-30.
SHIBUYA M.Vascular endothelial growth factor（VEGF）and its receptor（VEGFR）signaling in angiogenesis：a crucial target for anti- and pro-angiogenic therapies[J].Genes Cancer，2011,2(12):1097-1105.
CHEN M，CAI E，HUANG J，et al.Prognostic value of vascular endothelialgrowth factor expression in patients with esophageal cancer：a systematic review and meta-analysis[J].Cancer Epidemiol Biomarks Prev，2012,21(7):1126-1134.
王龙，戴天阳.VEGF和VM对食管鳞状上皮癌血液供应的影响及临床意义[J].重庆医学，2014,43(11):1380-1382.
LI C H，SUN X J，NIU S S，et al.Overexpression of IQGAP1 promotes the angiogenesis of esophageal squamous carcinoma through the AKT and ERK-mediated VEGF-VEGFR2 signaling pathway[J].Oncol Rep，2018,40(3):1795-1802.
SHI Q T，DIAO Y L，JIN F，et al.Anti-metastatic effects of Aidi on human esophageal squamous cell carcinoma by inhibiting epithelial-mesenchymal transition and angiogenesis[J].Mol Med Rep，2018,18(1):131-138.
LI N，LU Y J，LI D M，et al.All-trans retinoic acid suppresses the angiopoietin-Tie2 pathway and inhibits angiogenesis and metastasis in esophageal squamous cell carcinoma[J].PLoS One，2017,12(4):e0174555.
赵美，刘峰，王炯轶，等.JAK2/STAT3信号通路对非小细胞肺癌血管生成的影响[J].中华医学杂志，2011,91(6):375-381.
傅军，傅玉春，张晓雲，等.JAK2/STAT3信号通路对胃癌血管生成的影响[J].蚌埠医学院，2019,44(6):701-704.
潘立峰，单保恩，郑宝军，等.血管内皮生长因子反义RNA对裸鼠食管癌细胞增殖和凋亡的影响意义[J].肿瘤，2007,27(1):9-12.
潘立峰，李巧霞，单保恩，等.VEGF反义RNA抑制裸鼠体内食管癌细胞生长的实验研究[J].肿瘤防治研究，2005,32(8):457-459.
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