针刺调控Klotho表达对SHR大鼠蛋白尿及肾脏病理改善的实验研究
Effect of acupuncture regulating Klotho expression on proteinuria and renal pathology in SHR rats
- 上海中医药杂志 2021年55卷第4期 页码：73-78
- 作者机构：
1.上海中医药大学附属曙光医院（上海 200021）
2.上海市奉贤区南桥镇社区卫生服务中心（上海 201408）
3.上海市光华中西医结合医院（上海 200052）
4.上海中医药大学附属第七人民医院（上海 200137）
5.上海市黄浦区香山中医医院（上海 200020）；
- 作者简介：
宁思思，女，硕士，住院医师，主要从事中西医结合防治慢性肾脏病研究工作
李屹，主任医师，硕士研究生导师；E-mail:liyi_1313@126.com
- 基金信息：上海中医药大学预算内项目(ZDCG201704);上海市名老中医学术经验研究工作室项目(SHGZS-2017027)
DOI：10.16305/j.1007-1334.2021.2004151
中图分类号：
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宁思思, 杨绍政, 王嘉琳, 等. 针刺调控Klotho表达对SHR大鼠蛋白尿及肾脏病理改善的实验研究[J]. 上海中医药杂志, 2021,55(4):73-78.
Sisi NING, Shaozheng YANG, Jialin WANG, et al. Effect of acupuncture regulating Klotho expression on proteinuria and renal pathology in SHR rats[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(4):73-78.
宁思思, 杨绍政, 王嘉琳, 等. 针刺调控Klotho表达对SHR大鼠蛋白尿及肾脏病理改善的实验研究[J]. 上海中医药杂志, 2021,55(4):73-78. DOI： 10.16305/j.1007-1334.2021.2004151.
Sisi NING, Shaozheng YANG, Jialin WANG, et al. Effect of acupuncture regulating Klotho expression on proteinuria and renal pathology in SHR rats[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(4):73-78. DOI： 10.16305/j.1007-1334.2021.2004151.
摘要
目的,2,观察针刺对自发性高血压模型大鼠（SHR）蛋白尿及病理的影响。,方法,2,将SHR大鼠随机分为模型组、氯沙坦组、肾俞组、膈俞组、肾俞+膈俞组，每组10只，另以10只WKY大鼠作为正常组。氯沙坦组予氯沙坦钾片溶于0.9%NaCl溶液，按30 mg·kg,-1,·d,-1,灌胃，每日1次；肾俞组、膈俞组、肾俞+膈俞组在相应腧穴予电针干预，隔日1次；模型组做与针刺组同等固定，不予针刺。各组均连续干预12周。分别于干预前、干预第4、8、12周末以生化法检测大鼠24 h尿蛋白定量，12周末取大鼠肾脏标本以光镜观察HE染色、电镜观察肾脏超微结构的病理变化，免疫组化技术检测肾组织Klotho蛋白的阳性表达面积。,结果,2,干预结束后，氯沙坦组及针刺各组24 h尿蛋白均低于模型组（,P,<,0.05）；氯沙坦组及针刺各组的肾脏病理损伤均有不同程度减轻；与模型组比较，氯沙坦组、针刺各组Klotho阳性面积表达均有所升高（,P,<,0.05）。,结论,2,针刺可通过调节肾脏Klotho的表达，进而减少SHR大鼠的蛋白尿、减轻肾脏病理损伤，延缓高血压肾损害进展。
Abstract
Objective,2,To observe the effect of acupuncture on proteinuria and renal pathology in spontaneous hypertension rats (SHR).,Methods,2,SHR rats were randomly divided into model group, losartan group, Shenshu acupuncture group, Geshu acupuncture group, and Shenshu + Geshu group, with 10 rats in each group, and 10 WKY rats were set as normal group. The losartan group was intragastrically administered with losartan tablets which were dissolved in normal saline, with 30 mg/kg once a day. The acupuncture groups were given with electro-acupuncture intervention according to the corresponding acupoints every other day. The model group was fixed like the acupuncture groups done and without acupuncture. All groups were intervened continuously for 12 weeks. 24-hour urinary protein was detected by biochemical method before intervention and at the end of 4th, 8th and 12th week of intervention. At the end of 12th week, kidney samples were taken to observe the pathological changes of renal ultrastructure by light microscope and electron microscope, and the positive expression area of Klotho protein in renal tissue was detected by immunohistochemistry.,Results,2,After intervention, the 24 h urinary protein in the losartan group and the acupuncture groups were lower than that in the model group(,P,<, 0.05). The renal pathological injury in the losartan group and the acupuncture groups were alleviated in varying degrees, and the expression of Klotho positive area in the losartan group and the acupuncture groups were higher than that in the model group (,P,<,0.05).,Conclusion,2,Acupuncture can regulate the expression of Klotho in kidney, reduce proteinuria and renal pathological damage in SHR rats, and delay the progression of hypertensive renal damage.
关键词
自发性高血压大鼠针刺Klotho蛋白尿肾脏病理
Keywords
spontaneous hypertensionratsacupunctureKlothoproteinuriarenal pathology
references
杨雪军，刘飞，吴中平. 麻黄连翘赤小豆汤治疗IgA肾病大鼠的实验研究[J]. 上海中医药杂志，2017, 51(3): 76-79.
陈宝琅，李睿. 氯沙坦对自发性高血压大鼠肾脏组织Bax、Bcl-2表达的影响情况[J]. 中国循证心血管医学杂志，2016, 8(8): 965-967.
甯海燕，唐成林，周裕婷，等. 电针刺激对自发性高血压肾病大鼠血压及血清胱抑素C的影响[J]. 内蒙古中医药，2014, 33(1): 102-104.
郭义. 实验针灸学[M]. 北京：中国中医药出版社，2016: 107.
杨绍政，宁思思，孙玉霞，等. 针刺对自发性高血压大鼠肾脏纤维化相关指标的影响[J].针刺研究，2019, 44(12): 911-915.
宁思思，杨绍政，马家怡，等. 电针对自发性高血压大鼠肾脏Klotho蛋白表达及氧化应激的影响[J]. 中华中医药杂志，2019, 34(8): 3739-3742.
田园，李洁. 补肾活血汤干预老年高血压肾虚血瘀证临床研究[J]. 中国中医药信息杂志，2015, 22(2): 25-27.
安安，高建东. 浅谈益肾活血法治疗原发性高血压早期肾损害体会[J]. 内蒙古中医药，2013, 32(25): 10.
孙静文，王朝阳，田艳鹏，等. 针刺捻转补泻手法对SHR大鼠肾脏TGF-β1表达的影响[J].中华中医药学刊，2015, 33(9): 2123-2126.
刘健，高婷，万基伟，等. 针刺对自发性高血压大鼠血压的影响及其抗氧化应激机制研究[J]. 天津中医药，2015, 32(8): 488-491.
董爱爱，郭继龙，崔依依，等. 针刺对自发性高血压大鼠血管紧张素Ⅱ及其受体1以及γ-氨基丁酸的影响[J]. 中国中医急症，2018, 27(11): 1891-1894.
严冬，钱玉良，唐蜀华，等. 降压益肾颗粒对SHR大鼠早期肾脏损害组织形态学的影响[J]. 中国中医药科技，2004, 11(4): 210-211.
梁秀玲，邢燕. 肾素-血管紧张素系统的阻断及其对肾脏的保护作用[J]. 新乡医学院学报，2003, 20(2): 110-113.
王杰，陈艳琼，何凯杰，等. 从肾络亏虚理论探讨肾脏纤维化的病机与治疗策略[J]. 世界最新医学信息文摘，2017, 17(99): 98-99.
王海燕. 肾脏病学[M]. 3版. 北京：人民卫生出版社，2008: 437.
王建波，曲怡，张立德. 电针对高血压前期Dah l大鼠肾脏CaM/NO通路的影响[J]. 上海针灸杂志，2017, 36(11): 1367-1371.
郭骐影，孙启胜，贾文睿，等. 针刺对应激性高血压前期大鼠肾脏基因表达的影响[J].长春中医药大学学报，2016, 32(6): 1112-1115.
陈志娟，曲怡，刘宇，等. 电针刺激曲池、足三里穴对自发性高血压大鼠血压和肾功能的影响[J]. 中国中医急症，2012, 21(9): 1423-1424.
XU Y, SUN Z. Molecular basis of Klotho: from gene to function in aging[J]. Endocr Rev, 2015, 36(2): 174-193.
ASAI O, NAKATANI K, TANAKA T, et al. Decreased renal alpha-Klotho expression in early diabetic nephropathy in humans andmice and its possible role inurinary calcium excretion[J]. Kidney Int, 2012, 81(6): 539-547.
AKIMOTO T, YOSHIZAWA H, WATANABE Y, et al. Characteristics of urinary and serum soluble Klothoprotein in patients with different degrees of chronic kidney disease[J]. BMC Nephrology, 2012, 13(1): 155.
HARUNA Y, KASHIHARA N, SATOH M, et al. Amelioration of progressive renal injury by genetic manipulation of Klotho gene[J]. Proc Natl Acad Sci USA, 2007, 104(7): 2331-2336.
YANG K, WANG C, NIE L, et al. Klotho protects against indoxyl sulphate-induced myocardial hypertrophy[J]. J Am Soc Nephrol, 2015, 26(10): 2434-2446.
WANG Y, SUN Z. Antiaging gene Klotho regulates endothelin-1levels and endothelin receptor subtype B expression in kidneys of spontaneously hypertensive rats[J]. J Hypertens, 2014, 32(8): 1629-1636.
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