矢志方减轻高尿酸血症大鼠肾小管损伤的作用机制研究
Study on protective effect and mechanism of Shizhifang Formula on renal tubular injury in hyperuricemia rats
- 上海中医药杂志 2021年55卷第7期 页码：85-90
- 作者机构：
1.上海中医药大学附属曙光医院肾病科，上海中医药大学中医肾病研究所，上海中医药大学肝肾疾病病证教育部重点实验室，上海市中医临床重点实验室（上海 201203）
2.上海中医药大学门诊部（上海 201203）
- 作者简介：
李瑞玲，女，主治医师，主要从事中医药治疗慢性肾脏病研究工作
高建东，教授，主任医师，博士研究生导师；E-mail：jiandong.gao@shtcm.edu.cn
- 基金信息：国家自然科学基金资助项目(81373613;81874437;81904126)
DOI：10.16305/j.1007-1334.2021.2004056
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李瑞玲, 吴燕升, 李东东, 等. 矢志方减轻高尿酸血症大鼠肾小管损伤的作用机制研究[J]. 上海中医药杂志, 2021,55(7):85-90.
Ruiling LI, Yansheng WU, Dongdong LI, et al. Study on protective effect and mechanism of Shizhifang Formula on renal tubular injury in hyperuricemia rats[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(7):85-90.
李瑞玲, 吴燕升, 李东东, 等. 矢志方减轻高尿酸血症大鼠肾小管损伤的作用机制研究[J]. 上海中医药杂志, 2021,55(7):85-90. DOI： 10.16305/j.1007-1334.2021.2004056.
Ruiling LI, Yansheng WU, Dongdong LI, et al. Study on protective effect and mechanism of Shizhifang Formula on renal tubular injury in hyperuricemia rats[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(7):85-90. DOI： 10.16305/j.1007-1334.2021.2004056.
摘要
目的,2,探索矢志方减轻高尿酸血症大鼠肾小管损伤的作用机制。,方法,2,32只大鼠按体质量随机分为正常组、模型组、矢志方组、别嘌醇组，每组8只；以750 mg/kg 氧嗪酸钾造模，各组分别于实验的第3、5、7、11周末留取10只大鼠24 h尿液，检测24 h尿蛋白定量、尿β-N-乙酰氨基葡萄糖苷酶（NAG）、尿视黄醇结合蛋白（RBP）水平；免疫组化法、Real-time PCR、Western blot检测Ⅲ型胶原（Collagen Ⅲ）和骨桥蛋白（OPN）的表达。,结果,2,第11周末，模型组NAG水平显著高于正常组（,P,<,0.05），RBP水平较正常组在第5、7、11周末均有不同程度升高（,P,<,0.05），用药后可降低尿中RBP排泄。矢志方组、别嘌醇组,collagen ,Ⅲ,、,OPN, mRNA的表达自3周起较模型组升高（,P,<,0.01，,P,<,0.05），两组各时间节点,OPN, mRNA的表达无差异（,P,>,0.05）。与模型组比较，各时间段各组大鼠肾组织中collagen Ⅲ表达明显降低（,P,<,0.05）；除第3周外，各组大鼠肾组织中OPN表达明显降低（,P,<,0.01）。第3周末，矢志方组、别嘌醇组之间collagen Ⅲ表达无差异（,P,>,0.05）；第3、7周末两组之间OPN表达无差异（,P,>,0.05）。免疫组化可见模型组collagen Ⅲ和OPN表达于肾小管及间质，肾小球在模型后期有少量表达。,结论,2,高尿酸血症大鼠肾小管微量蛋白排泄增多，高尿酸血症存在明显的肾小管和间质损伤，矢志方具有减轻肾小管损伤、抑制肾脏间质炎症、缓解肾纤维化的作用。
Abstract
Objective,2,To explore the protective effect and mechanism of Shizhifang Formula on renal tubular injury in hyperuricemia rats.,Methods,2,Thirty two rats were randomly divided into 4 groups according to their body weight: normal group, model group, Shizhifang Formula group and allopurinol group, 8 rats in each group. The rats were given 750 mg/kg OA suspension to establish hyperuricemic model. At the end of the 3rd, 5th, 7th and 11th week of the experiment, 24-hour urine was taken in each group with 10 rats, and 24-hour urine protein quantification, renal tubular function index NAG and RBP were further detected. Immunohistochemistry, real time PCR and Western blot were used to detect the expression of ,collagen, Ⅲ and osteopantin(OPN).,Results,2,At the end of the 11th week, NAG in the model group was significantly higher than that in the normal group (,P,<, 0.05) . RBP in hyperuricemic rats was higher than that in normal rats in 5 weeks, 7 weeks and 11 weeks (,P,<,0.05) . Compared with the allopurinol group, the expression of ,collagen ,Ⅲ,and ,OPN, mRNA in Shizhifang Formula group and allopurinol group increased significantly (,P,<,0.01,P,<,0.05) from three weeks, and there was no difference in the expression of ,OPN, mRNA between the two groups at each time node (,P,>,0.05) . Compared with the model group, the expression of collagen Ⅲ in renal tissue of rats in the normal group was decreased significantly (,P,<,0.05) . Except for 3 weeks, the expression of OPN in renal tissue of rats in each group decreased significantly (,P,<,0.01) . There was no difference in the expression of collagen Ⅲ between the two treatment groups at the end of the 3th week (,P,>,0.05) , and there was no difference in the expression of OPN between the two treatment groups at the end of the 3th week and the 7 the week (,P,>,0.05) . The expression of collagen Ⅲ and OPN in renal tubules and interstitium in the model group was observed by immunohistochemistry, and there was a little expression in glomerulus in the later stage of the model.,Conclusions,2,In this study, the excretion of urinary microproteins in hyperuricemia rats was significantly increased, suggesting that there was obvious damage to renal tubules and interstitium in hyperuricemia. Shizhifang Formula has the effect of inhibiting renal interstitial inflammation and alleviating renal fibrosis.
关键词
矢志方高尿酸血症肾小管损伤模型大鼠中药研究
Keywords
Shizhifang Formulahyperuricemiakidney tubules injury mice modelresearch of traditlonal Chinese herbal medicine
references
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