醒神解郁汤对卒中后抑郁模型大鼠Th1/Th2及BDNF水平的影响
Effects of Xingshen Jieyu Decoction on levels of Th1/Th2 and BDNF in rats with post-stroke depression
- 上海中医药杂志 2021年55卷第3期 页码：87-92
- 作者机构：
1.上海中医药大学附属第七人民医院神经内科神经康复科（上海 200120）
2.复旦大学附属华山医院神经内科（上海 200040）
3.上海中医药大学附属岳阳中西医结合医院急诊科（上海 200080）
4.复旦大学附属华山医院北院中西医结合科（上海 201907）
5.上海中医药大学附属第七人民医院健康管理部（上海 200120）
6.上海市浦东新区疾病预防控制中心（上海 200136）
- 作者简介：
韩振翔，男，副主任医师，副教授，主要从事中西医结合脑卒中临床和基础研究工作
周一心，主任医师，硕士研究生导师；E-mail:asadezh@sina.com.
- 基金信息：国家自然科学基金项目(81703997);上海市浦东新区海派中医流派浦东分基地建设项目(PDZY-2019-0703);上海市中医优势病种培育项目(ZY〔2018-2020〕-ZYBZ-16);上海市中医诊疗模式创新试点建设项目(ZY〔2018-2020〕-FWTX-6015);上海中医药大学高峰高原创新团队资助项目(ZY3-RCPY-1-1001);上海市浦东新区领先人才项目(PWR12016-02);全国中医药创新骨干人才培训项目(2019)
DOI：10.16305/j.1007-1334.2021.2002033
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韩振翔, 侯臻臻, 韩翔, 等. 醒神解郁汤对卒中后抑郁模型大鼠Th1/Th2及BDNF水平的影响[J]. 上海中医药杂志, 2021,55(3):87-92.
Zhenxiang HAN, Zhenzhen HOU, Xiang HAN, et al. Effects of Xingshen Jieyu Decoction on levels of Th1/Th2 and BDNF in rats with post-stroke depression[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(3):87-92.
韩振翔, 侯臻臻, 韩翔, 等. 醒神解郁汤对卒中后抑郁模型大鼠Th1/Th2及BDNF水平的影响[J]. 上海中医药杂志, 2021,55(3):87-92. DOI： 10.16305/j.1007-1334.2021.2002033.
Zhenxiang HAN, Zhenzhen HOU, Xiang HAN, et al. Effects of Xingshen Jieyu Decoction on levels of Th1/Th2 and BDNF in rats with post-stroke depression[J]. Shanghai Journal of Traditional Chinese Medicine, 2021,55(3):87-92. DOI： 10.16305/j.1007-1334.2021.2002033.
摘要
目的,2,研究醒神解郁汤对卒中后抑郁（PSD）大鼠血清Th1/Th2及脑源性神经营养因子（BDNF）水平的影响，探讨其可能的作用机制。,方法,2,SD大鼠随机分为正常组、卒中组、卒中后抑郁组、舍曲林组、醒神解郁汤低剂量组、醒神解郁汤高剂量组。其中，卒中组建立右侧大脑中动脉缺血再灌注（MCAO）模型；MCAO模型术后恢复3 d后加慢性不可预测的轻度应激（CUMS）法建立卒中后抑郁模型，PSD造模成功后应用舍曲林（5.142 g/kg）或醒神解郁汤高、低剂量（56.148 g/kg、18.716 g/kg）干预。采用酶联免疫吸附试验法（ELISA）检测大鼠血清炎症因子Th1（IL-6、IFN-γ）、Th2（IL-4、IL-10）、BDNF含量。,结果,2,与正常组比较，卒中后抑郁组血清Th1/Th2比值明显增加，抗炎因子IL-4、IL-10以及BDNF含量显著下降，促炎因子IL-6、IFN-γ含量显著增加，差异有统计学意义（,P,<,0.05）。与卒中后抑郁组比较，醒神解郁汤低剂量组、高剂量组血清Th1/Th2比值明显下降，IL-4、IL-10、BDNF含量明显增加，IL-6、IFN-γ含量明显降低，差异有统计学意义（,P,<,0.05）。,结论,2,醒神解郁汤可通过调节血清炎症因子Th1/Th2及BDNF水平，改善卒中后抑郁大鼠的神经功能及抑郁行为。
Abstract
Objective,2,To study the effect of Xingshen Jieyu Decoction on serum Th1/Th2 and brain-derived neurotrophic factor(BDNF) levels in post-stroke depressive (PSD) rats, and to analyze the possible mechanism of Xingshen Jieyu Decoction in treating the disease.,Methods,2,SD rats were randomly divided into normal group, stroke group, post-stroke depression group, sertraline group, low-dose group of Xingshen Jieyu Decoction, and high-dose group of Xingshen Jieyu Decoction. The stroke model was established by the right middle cerebral artery ischemia-reperfusion operation. The chronic unpredictable mild stress (CUMS) method was added at 3 days after the middle cerebral artery occlusion (MCAO) operation for PSD model. In the medicine groups the sertraline (5.142 g/kg) and high or low dose (56.148 g/kg, 18.176 g/kg) of Xingshen Jieyu Decoction were used after the establishment of PSD model respectively. The enzyme-linked immunosorbent assay (ELISA) was used to detect the contents of serum inflammation factors including Th1 (IL-6, IFN-γ), Th2 (IL-4, IL-10) and BDNF.,Results,2,Compared with those in the normal group, the serum Th1/Th2 ratio in the post-stroke depression group increased significantly, the levels of anti-inflammatory factors such as IL-4, IL-10, and BDNF decreased significantly, and the levels of pro-inflammatory factors such as IL-6 and IFN-γ increased significantly (,P,<,0.05); compared with those in the post-stroke depression group, the serum Th1/Th2 ratio was decreased significantly and the contents of IL-4, IL -10 and BDNF were increased significantly, but the contents of IL-6 and IFN-γ were decreased significantly (,P,<,0.05) in the post-stroke depression groups with low-dose, high-dose of Xingshen Jieyu Decoction.,Conclusion,2,Xingshen Jieyu Decoction can improve the neurological function and depressive behavior of post-stroke depression rats by regulating the levels of Th1 / Th2 and BDNF.
关键词
卒中后抑郁大鼠醒神解郁汤Th1/Th2脑源性神经营养因子
Keywords
post-stroke depressionratsXingshen Jieyu DecoctionTh1/Th2brain derived neurotrophic factor
references
VILLA R F, FERRARI F, MORETTI A. Post-stroke depression: mechanisms and pharmacological treatment[J]. Pharmacol Ther, 2018(184): 131-144.
BOIKO A S, LOSENKOV I S, LEVCHUK L A, et al. Biomarkers of depressive disorders: a multiplex analysis of blood serum[J]. European Psychiatry, 2017, 41(S1): S524.
CHENG C, LIU X, FAN W, et al. Comprehensive rehabilitation training decreases cognitive impairment, anxiety, and depression in poststroke patients: a randomized, controlled study[J]. J Stroke Cerebrovasc Dis, 2018, 27(10): 2613-2622.
GRUNDMANN O, LV Y, KELBER O, et al. Mechanism of St. John’s wort extract (STW3-VI) during chronic restraint stress is mediated by the interrelationship of the immune, oxidative defense, and neuroendocrine system[J]. Neuropharmacology, 2010, 58(4-5): 767-773.
MYINT A M, LEONARD B E, STEINBUSCH H W, et al. Th1, Th2, and Th3 cytokine alterations in major depression[J]. J Affect Disord, 2005, 88(2): 167-173.
周一心，韩振翔. 醒神解郁汤治疗脑卒中后轻度抑郁30例疗效观察[J]. 新中医,2015, 47(11): 35-37.
LIU F, SCHAFER D P, MCCULLOUGH L D. TTC, fluoro-Jade B and Neu N staining confirm evolving phases of infarction induced by middle cerebral artery occlusion[J]. J Neurosci Methods, 2009, 179(1): 1-8.
ZHU K Y, MAO Q Q, IP S P, et al. A standardized Chinese herbal decoction, kai-xin-san, restores decreased levels of neurotransmitters and neurotrophic factors in the brain of chronic stress-induced depressive rats[J/OL].Evid Based Complement Alternat Med, 2012[2020-02-01]. https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437946/https://www.ncbi.nlm.nih.gov/pmc/articles/PMC3437946/.
ZAUSINGER S, HUNGERHUBDER E, BAETHMANN A, et al. Neurological impairment in rats after transient middle cerebral artery occlusion: a comparative study under various treatment paradigms[J]. Brain Res, 2000, 863(1-2): 94-105.
徐叔云，卞如濂，陈修. 《药理实验方法学》第二版出版发行[J]. 中国药理学通报,1992(1): 19.
李翠娟，巩振东. 从脾论治情志病探析[J]. 时珍国医国药,2018, 29(4): 930-931.
窦志芳，张俊龙，赵琼. 脑脾相关理论研究[J]. 山西中医学院学报,2015, 16(4): 6-7.
BELINGA V F, WU G J, YAN F L, et al.Splenectomy following MCAO inhibits the TLR4-NF-κB signaling pathway and protects the brain from neurodegeneration in rats[J]. J Neuroimmunol, 2016(293): 105-113.
CHAUHAN A, AL MAMUN A, SPIEGEL G, et al. Splenectomy protects aged mice from injury after experimental stroke[J]. Neurobiol Aging, 2018(61): 102-111.
于路航. 石菖蒲对卒中后抑郁大鼠模型血清脑源神经营养因子和血管内皮生长因子的影响[D].保定：河北大学，2018.
许亚萍，伍旭明. 石菖蒲-远志药对配伍机制初步探讨[J]. 中华中医药杂志,2018, 33(12): 5629-5632.
左军，牟景光，胡晓阳. 半夏化学成分及现代药理作用研究进展[J]. 辽宁中医药大学学报,2019, 21(9): 26-29.
祝洪艳，何忠梅，王国丽，等. 参芪五味子片对神经系统的药理作用及临床研究[J]. 吉林中医药,2014, 34(8): 842-844.
俞春林，杜正彩，郝二伟，等. 四类不同功效桂枝药对化学成分与药理作用的研究进展[J]. 中国实验方剂学杂志,2020, 26(1): 226-234.
胡立宏，房士明，刘虹，等. 蒲黄的化学成分和药理活性研究进展[J]. 天津中医药大学学报,2016, 35(2): 136-140.
胡静娜，马卫成，李越兰. 半夏秫米汤对慢性应激抑郁模型小鼠行为学及脑内单胺类神经递质含量的影响[C]//浙江省医学会临床药学分会. 2016年临床药学高峰论坛暨浙江省医学会临床药学分会学术会议论文汇编. 宁波：浙江省科学技术协会，2016.
CAI W, MUELLER C, LI Y J, et al. Post stroke depression and risk of stroke recurrence and mortality: a systematic review and meta-analysis[J]. Ageing Res Rev, 2019(50): 102-109.
张一清，朱晓军，王彤.脑卒中后抑郁症与下丘脑-垂体-肾上腺轴的关系及运动干预的研究进展[J].中国康复医学杂志,2014, 29(5): 492-494.
BERGAMINI G, MECHTERSHEIMER J, AZZINNARI D, et al. Chronic social stress induces peripheral and central immune activation, blunted mesolimbic dopamine function, and reduced reward-directed behaviour in mice[J]. Neurobiol Stress, 2018(8): 42-56.
GUAN S Z, LIU J W, FANG E F, et al. Chronic unpredictable mild stress impairs erythrocyte immune function and changes T-lymphocyte subsets in a rat model of stress-induced depression[J]. Environ Toxicol Pharmacol, 2014, 37(1): 414-422.
DUDA W, CURZYTEK K, KUBERA M, et al. Interaction of the immune-inflammatory and the kynurenine pathways in rats resistant to antidepressant treatment in model of depression[J]. Int Immunopharmacol, 2019(73): 527-538.
HERKENHAM M, KIGAR S L. Contributions of the adaptive immune system to mood regulation: mechanisms and pathways of neuroimmune interactions[J]. Prog Neuropsychopharmacol Biol Psychiatry, 2017, 79(Pt A): 49-57.
马涛，高东阳，于姚，等. 慢性应激对大鼠脾脏能量代谢及细胞免疫功能损伤和醒脾解郁法的干预机制研究[J]. 环球中医药,2019, 12(3): 321-327.
DUMAN R S, MONTEGGIA L M. A neurotrophic model for stress-related mood disorders[J]. Biol Psychiatry, 2006, 59(12): 1116-1127.
CHEN X Q, LI C F, CHEN S J, et al. The antidepressant-like effects of Chaihu Shugan San: dependent on the hippocampal BDNF-TrkB-ERK/Akt signaling activation in perimenopausal depression-like rats[J]. Biomed Pharmacother, 2018(105): 45-52.
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