Objective:To study the feasibility of zebrafish as a drug screening model for the treatment of obesity and the effect of Jianpi Shugan Jiangzhi recipe on lipid metabolism of obese zebrafish. MethodsZebrafish fertilized for 14 days were divided into control group, model group(0 mg/L), high-dose group(1 600 mg/L), medium-dose group(400 mg/L)and low-dose group(100 mg/L).The control group was normally fed, and the other four groups were overfed. After overfeeding, the zebrafish were soaked in the corresponding concentration of medicinal solution for 8 h per day.After 56 days of drug intervention, the body length and mass of zebrafish were measured to calculate the fullness. The concentration of triglyceride and total cholesterol as well as the degree of histological degeneration and fat accumulation in the liver of zebrafish were detected.  Results:The mass, fullness and the concentration of triglyceride of zebrafish in the model group were higher than those of the control group(P<0.05), while the indexes of the zebrafish in the high-dose group were significantly lower than those in the model group(P<0.05).The concentration of triglyceride in the medium-dose group was significantly lower than that in the model group(P<0.05). The liver histological degeneration and fat accumulation in the model group were obvious. The histological degeneration and fat accumulation of zebrafish in the Jianpi Shugan Jiangzhi recipe group were less than those in the model group.  Conclusion:Zebrafish can be successfully used to establish obesity model and Jianpi Shugan Jiangzhi recipe can effectively reduce the fat level of zebrafish.