Objective:To observe the clinical efficacy of Bushen Huoxue decoction in the treatment of premature ovarian failure(POF)and explore its influence on the relevant immune antibody. MethodsEighty-two patients of POF were enrolled and randomly divided into the Chinese medicine group and the western medicine group,41 cases in each group.The patients in the Chinese medicine group were orally treated with Bushen Huoxue Decoction,and the patients in the western medicine group were orally treated with sequential therapy of progynova combined with medroxyprogesterone acetate.The treatment course of the two groups was 6 months. Before and after treatment, the Chinese medical syndrome scores of the two groups were compared; the levels of follicle-stimulating hormone(FSH), luteinizing hormone(LH), estradiol(E,2,), prolactin(PRL), progesterone(P), testosterone(T), anti-mullerian hormone(AMH), antinuclear antibody(ANA), anticardiolipin antibody(ACA)and anti-ovarian antibody(AOA)were detected; the uterine volume, ovarian volume, and the peak systolic velocity(PSV)and resistance index(RI)of ovarian stromal blood flow in the two groups were measured and compared through ultrasonic examination.  Results:In the course of treatment, 4 cases in the Chinese medicine group and 2 cases in the western medicine group were eliminated, and finally 37 cases in the Chinese medicine group and 39 cases in the western medicine group were included in the statistical analysis.①After treatment, the Chinese medical syndrome scores of the two groups were significantly decreased(P,<,0.01), and the scores of the Chinese medicine group were lower than those of the western medicine group(P,<,0.01).②=2\*GB3After treatment, the level of FSH and the radio of FSH/LH in the Chinese medicine group were significantly decreased(P,<,0.05), the level of FSH in the western medicine group was also decreased(P,<,0.05), and there were no statistical differences on the levels of sex hormones between the two groups(P,>,0.05).③=3\*GB3After treatment, the levels of AMH in the two groups were increased(P,<,0.05), the levels of ANA and AOA were decreased(P,<,0.05), and the level of AMH in the Chinese medicine group was higher than that in the western medicine group and the level of ANA in the Chinese medicine group was lower than that in the western medicine group(P,<,0.05).④=4\*GB3After treatment, the ovarian volume and the PSV of ovarian stromal blood flow in the two groups were increased compared with treatment before(P,<,0.05), the RI in the Chinese medicine group was decreased compared with treatment before(P,<,0.05), and the RI in the Chinese medicine group was lower than that in the western medicine group(P,<,0.05).  Conclusion:Bushen Huoxue decoction can effectively improve the Chinese medical syndrome of the patients with POF, down-regulate the level of FSH and the ratio of FSH/LH, up-regulate the level of AMH, regulate the ANA-ACA-AOA pathway, thereby balance the hypothalamus-hypophysis-gonad axis, alleviate the damages of ovarian granulosa cells and follicle, and relieve POF.