Objective:To explore the relationship between the active components and targets of Xuebijing injection and coronavirus disease 2019 (COVID-19), and to provide theoretical basis for Xuebijing injection in the treatment of COVID-19 and new drug development. MethodsThe active compounds of Xuebijing injection and corresponding targets of each compounds were obtained from the TCMSP database and published researches, and the network was constructed and analyzed by Cytoscape. GO and KEGG analyses were performed on the obtained targets by DAVID and STRING databases.  Results:①=1\*GB3From the database and published researches, 22 compounds in Xuebijing injection were obtained, corresponding to 504 potential targets. ②Among them 70 proteins were intersected with targets co-expressed with angiotensin-converting enzyme 2 (ACE2), a receptor of the novel coronavirus, suggesting that Xuebijing injection may play a therapeutic role on COVID-19 through these 70 proteins. ③=3\*GB3These target proteins regulate a series of signaling pathways, such as HIF-1 signaling pathway and PI3K-Akt signaling pathway.  Conclusion:Xuebijing injection reflected an overall regulation of multi-components and multi-targets of traditional Chinese medicine for the treatment of COVID-19. It may realize the anti-virus function by regulating the pathways related to the development of disease through 70 proteins that can interact with ACE2 to relieve inflammation and inhibit virus replication.