Objective:To improve the quality standard of Erkeling syrup. MethodsBulbus Fritillariae Thunbergii was identified by TLC.The contents of ephedrine hydrochloride,amygdalin and baicalin were analyzed by HPLC on Kromasil C,18, column (250 mm × 4.6 mm,5 μm) with the mobile phase of acetonitrile-0.1% phosphate by gradient elution.The flow rate was 1.0 mL/min and the detection wavelength was set at 210 nm for ephedrine hydrochloride and amygdalin,280 nm for baicalin. The column temperature was 30 ℃. RseultsTLC identification method of Bulbus Fritillaria thunbergii had strong specificity. The spots in TLC were clear, and the negative samples showed no interference. Ephedrine hydrochloride had a good linear relationship with peak area in the range of 1.050 to 10.500 mg/L (r=0.999 4), and the average recovery rate was 99.1%(RSD=1.50%, n=9). In the range of 5.220-52.20mg/L, amygdalin had a good linear relationship with peak area (r=0.999 0), and the average recovery rate was 98.6%(RSD=0.81%, n=9). Baicalin showed a good linear relationship with peak area in the range of 20.050-200.500 mg/L (r=0.999 5), with an average sample recovery of 98.4%(RSD=0.94%, n=9).  Conclusion:The method is accurate and repeatable,and can be used as the quality control of Erkeling syrup.