Objective:To observe the effect and mechanism of Fugan formula on regulating miR-424 expression to inhibit the angiogenesis of human hepatic sinusoidal endothelial cells (HHSEC). MethodsFirstly, Fugan formula extract was incubated with HHSEC in different doses (0～2 000 mg/L). The effect of Fugan formula on the cell activity was detected by CCK8 method, the range of the non-toxic effect of Fugan formula on the cell was analyzed and the dosage was used for the follow-up efficacy and mechanism evaluation. Then HHSEC were divided into the normal group,the CoCl,2, model group, the miR-424 inhibitor group and the Fugan formula group (200 mg/L). After 24 h incubation, cell viability and proliferation were detected by CCK8. Cell migration was observed by Transwell and tube formation was evaluated by Matrigel. The expression of miR-424, HIF-1α, vWF and CD31 were detected by RT-PCR and Western Blot.  Results:Fugan formula of 0~400 mg/L had no obvious cytotoxicity. Compared with the normal group, 200 μmol/L CoCl,2, can induce hypoxia injury model of HHSEC, and in the model group the expression of miR-424 and HIF-1 α increased, cell migration and lumen formation increased, and the expression of vWF and CD31 increased (P,<,0.01); Compared with the model group, after the intervention of miR-424 inhibitor and 200 mg/L Fugan formula, the expression of miR-424 and HIF-1 α decreased, the cell migration and tube formation were inhibited and the expression of vWF and CD31 decreased (P,<,0.01); and the comprehensive effect of Fugan formula was similar to that of miR-424 inhibitor.  Conclusion:Fugan formula could inhibit angiogenesis of HHSEC and its mechanism is related to the inhibition of miR-424 expression.