GE Xiuyun, DAI Guangxiu. Optimization of processing technology of charring chrysanthemum by orthogonal design[J]. Shanghai Journal of Traditional Chinese Medicine, 2019,53(12):78-82.
GE Xiuyun, DAI Guangxiu. Optimization of processing technology of charring chrysanthemum by orthogonal design[J]. Shanghai Journal of Traditional Chinese Medicine, 2019,53(12):78-82. DOI: 10.16305/j.1007-1334.2019.12.020.
Objective:To optimize the processing technology of charring chrysanthemum. MethodsCharred Chrysanthemum samples were prepared by means of L,9, (3,4,) orthogonal test design, and the contents of chlorogenic acid, luteolin, 3,5-O-di-coffee acyl quinic acid were determined by high performance liquid chromatography(HPLC). The gradient elution was carried out with acetonitrile as mobile phase A and 0.1% aqueous phosphoric acid as mobile phase B. The flow rate was 1.0 mL/ min, the detection wavelength was 348 nm, and the column temperature was 30 ℃. And the appearance character identification, the water content and the extract content determination were carried out. The optimum processing technology and the specific process parameters of charring chrysanthemum were selected based on the appearance character, the extract content and the content of the chlorogenic acid, the luteolin, the 3,5-O-di-coffee and the quinine acid. ResultThe optimum charring process of the chrysanthemum was as follows:1 000 g of chrysanthemum put in a hot pot with a temperature of 300 ℃, fried for 6 minutes. Conclusion:The optimized technology is stable,feasible and the technical paramater is definite.