Objective:To explore the effects of Huanglian(Rhizoma Coptidis)-Dahuang(Radix et Rhizoma Rhei)-Rougui(Cortex Cinnamomi)compound and its best components compatibility on tumor proliferation and syndromes of liver cancer mice. MethodsHuman hepatocarcinoma cells were injected subcutaneously into the right axilla to establish a liver cancer model of transplanted nude mice.After modeling success,mice were divided into model control group(model group),Huanglian(Rhizoma Coptidis)-Dahuang(Radix et Rhizoma Rhei)-Rougui(Cortex Cinnamomi)compound group(compound group) and berberine-emodin-cinnamaldehyde group (compatibility group). Each group was treated with corresponding drug for 2 weeks.During the administration, the state of the mice was observed.Before and after administration,the tumor diameter and volume,body weight, axillary temperature, grip force, number of horizontal movements and vertical standings in the squat field, microphotograph of the claws and tails were measured twice.The degree of excess or deficiency of Qi,Xue,Yin and Yang were calculated according to the above indexes.The ratios of post-treatment/pre-treatment of these indexes were used to analyze the change of syndromes.Finally, the mice were sacrificed and the tumors were weighed and the tumor inhibition rate was measured.  Results:①Mice state:During the experiment, the mice were all in good condition, both the movement and the defecation were normal.②Antitumor effect:The tumor volume of each group increased with time,which was displayed as the model group> compound group>compatibility group. The variation of tumor weight was consistent with that of the tumor volume.③Syndrome change:After treatment,the degree of excess or deficiency of Qi was increased,and the degree of the compatibility group was better than that of the compound group.The degree of excess or deficiency of Xue was also raised,and the degree of the compound group was superior to the compatibility group.The degree of excess or deficiency of Yin and Yang almost had no change.  Conclusion:Both the compound and the best compatibility of the medicines can inhibit the tumor proliferation of liver cancer mice and has the trend of invigorating qi and blood.