Objective:To investigate the effect of honeysuckle on the expression of Caspase-3 and NF-κB in mice with viral myocarditis. MethodsA total of 80 male BALB/c mice were divided into 4 groups randomly with 20 mice in each group:normal control group,model group,honeysuckle low-dose group and honeysuckle high-dose group. Coxsackievirus B3 (CVB3) was injected intraperitoneally into mice in model group and both dose groups to establish viral myocarditis model. The low and high dose groups were given honeysuckle liquid 30 minutes after virus injection, once a day for 14 days; the model group was given saline by gastric lavage; the control group was given intraperitoneal injection of Equivalent Eagle liquid without virus and normal saline by gastric lavage. Totally 15 mice were randomly selected and executed from each group on the 14th day. The pathological changes of myocardium were observed by HE staining, and the expression of Caspase-3, NF-κB in myocardial tissue was detected by immunohistochemistry.  Results:The expression of Caspase-3 and NF-κB in the model group was significantly higher than that in the control group. The expression of Caspase-3 and NF-κB in the two dose groups was significantly lower than that in the model group. The expression of Caspase-3 and NF-κB in the honeysuckle high-dose group was significantly lower than that in the honeysuckle low-dose group.  Conclusion:Honeysuckle can significantly inhibit the expression of Caspase-3 and NF-κB in mice with viral myocarditis, which provides the experimental basis for clinical application of honeysuckle in the treatment of viral myocarditis.