Objective:To study the effects of Astragalus Angelica Mixture combined with tacrolimus on immune and renal functions in patients with refractory nephrotic syndrome. Methods126 patients with refractory nephrotic syndrome were assigned into treatment group and control group, 63 patients in each group. Patients in both groups were given tacrolimus and symptomatic treatment, and those in the treatment group were treated with Astragalus Angelica Mixture in addition. The treatment course was 6 months. Clinical efficacy, changes in T lymphocyte subsets (CD4,+, CD8,+, CD4,+,/CD8,+,), serum creatinine (SCr), urea nitrogen (BUN), glomerular filtration rate (GFR), 24 h urine protein quantitation and serum albumin level, as well as the occurrence of adverse reactions were observed.  Results:①The total effective rate was 98.4% and 88.9% in the treatment group and the control group respectively; there were statistically significant differences in clinical efficacy between the groups (P,<,0.05). ②There were statistically significant differences in the levels of CD4,+, CD8,+, and CD4,+,/CD8,+, in both groups after treatment compared to those before treatment (P,<,0.05); after treatment, the level of CD8+ was lower in the treatment group than in the control group, and the levels of CD4+ and CD4,+,/CD8,+, were higher in the treatment group than in the control group, with statistically significant differences (P,<,0.05). ③The levels of SCr, BUN, 24 h urinary protein quantitation and serum albumin improved in both groups after treatment compared to those before treatment (P,<,0.05); after treatment, the levels of SCr, BUN and 24 h urinary protein quantitation were lower in the treatment group than in the control group, and the levels of serum albumin and GFR were higher in the treatment group than in the control group, with statistically significant differences (P,<,0.05). ④During the trial, the incidence of adverse reactions in the treatment group (11.11%) was significantly lower than that in the control group (31.74%) (P,<,0.05).  Conclusion:Astragalus Angelica Mixture combined with tacrolimus has superior clinical efficacy to tacrolimus alone in the treatment of refractory nephrotic syndrome, which significantly improves immune and renal functions and is safe.