Objective:To explore the mechanism of action of Feiyanning Recipe (FYN) in inhibiting invasion of the A549 lung cancer cells by regulating metastasis-associated lung adenocarcinoma transcript 1 (MALAT1). MethodsThe A549 cells transfected with LV-vector and LV-over/MALAT1 were treated with FYN (0～500 μg/ml). The expression of MALAT1 was detected by qRT-PCR; the effect of FYN on tumor cell proliferation was determined by MTT colorimetric assay; the invasive ability of tumor was determined by cell invasion test; and the expression of Wnt/β-catenin/EMT signaling pathway related proteins in A549 cells was detected by Western blotting.  Results:Results of the cell invasion test indicated that MALAT1 overexpression strengthened the invasive ability of the A549 cells, and there was significant difference compared to the normal control group and the lentivirus vector group (P<0.01). Compared to the normal control group, FYN inhibited invasion of the A549 cells, with significant difference (P<0.01), but the ability of FYN to inhibit cell invasion was reduced after MALAT1 overexpression.  Results: of MTT colorimetric assay showed that FYN inhibited proliferation of the A549 cells in a concentration-dependent manner.  Results: of qRT-PCR indicated that FYN down-regulated the expression of MALAT1.  Results: of Western blotting showed that MALAT1 down-regulated E-cadherin and up-regulated N-cadherin, Snail and β-catenin in the nucleus, whereas FYN up-regulated E-cadherin and down-regulated N-cadherin, Snail and β-catenin in the nucleus, but MALAT1 overexpression reduced the regulatory effect of FYN on these proteins.  Conclusion:MALAT1 overexpression promotes lung cancer cell invasion by activating the Wnt/β-catenin pathway and accelerating the EMT progress. Feiyanning Recipe inhibits invasion of the A549 lung cancer cells by down-regulating MALAT1 and suppressing the Wnt/β-catenin/EMT signal axis.