Objective:To explore the value of collagen fibers deposition, CD34 and Cytokeratin 19(CK19) expression in liver tissue in the diagnosis of chronic hepatitis B with blood stasis syndrome, in order to provide some reference for traditional Chinese medicine(TCM) syndrome differentiation and treatment on chronic hepatitis B.MethodsResearch objects were 128 hepatitis B patients undergoing liver biopsy.According to TCM four diagnostic information, the patients were divided into blood stasis syndrome group and non-blood stasis syndrome group.Hematoxylin-eosin staining, reticular fiber staining, Masson staining, CD34 and CK19 immunohistochemical staining were performed on the liver tissue sections, and then the positive expressions of collagen, CD34 and CK19 were calculated by the image analysis software to observe and compare the changes of collagen fiber, CD34 and CK19 expression in liver tissue.  Results:①The positive expressions of collagen fibers, CD34, and CK19 in patients with liver fibrosis stages S2, S3 and S4 were significantly than those in patients with S1 stage(P<0.05). The expression of collagen fibers and CD34 in patients with S4 stage were significantly higher than those in stages S2 and S3(P<0.05). ②The positive area of collagen fibers in G4 degree of inflammatory activity was significantly larger than those in G1, G2 and G3 degree (P<0.05). There was no significant difference between G1, G2 and G3 degree (P>0.05) and no significant difference in positive expression of CD34 and CK19 between different degrees of inflammatory activity (P>0.05).③There was a significant difference in fibrosis stages between patients with blood stasis syndrome and non-bloody stasis syndrome(P<0.05).The positive expression of collagen fiber and CD34 in patients with blood stasis syndrome was significantly higher than that in patients without blood stasis syndrome(P<0.05).  Conclusion:Positive expression of collagen fiber and CD34 in liver tissue of patients with chronic hepatitis B can be a quantitative index of liver fibrosis.Collagen fiber deposition and angiogenesis may be the main changes in liver histopathology of chronic hepatitis B with blood stasis syndrome, which may provide some references for TCM syndrome differentiation and treatment on chronic hepatitis B.