Objective:To investigate the regulatory effect of Chinese herbal compound for invigorating the kidney and removing dampness on the NALP6 signaling pathway in rats with gouty arthritis (GA). MethodsEighty-four SD rats were randomly assigned into the blank control group, model group, tongfengshu group, colchicine group, low-dose Chinese medicine group, medium-dose Chinese Medicine group and high-dose Chinese Medicine group. Gavage administration was performed once daily. At 1h after administration on day 7, the modified composite modeling method with potassium oxazinate and sodium urate was used to establish the GA rat model. Samples were collected at 4h after modeling, the pathological changes in synovial tissues were observed in rats of each group, the content of uric acid, caspase-1, IL-1β and IL-18 in serum was determined by ELISA and the expression level of NALP6 in synovial tissues was detected by Western blot.  Results:In the medium-dose Chinese medicine group treated with Chinese herbal compound for invigorating the kidney and removing dampness, the pathological changes in synovial tissues, the value of blood uric acid in the body, and the expression levels of caspase-1, IL-1β and IL-18 in serum decreased significantly compared to the model group (P<0.05); and in the high-dose Chinese medicine group treated with the Chinese herbal compound, Western blot analysis showed that the expression level of NALP6 decreased significantly in synovial tissues compared to the model group (P<0.05).  Conclusion:Blocking the inflammatory signaling pathway of “NALP6-caspase-1-IL-1β” is an important mechanism of action in preventing and treating gouty arthritis with Chinese herbal compound for invigorating the kidney and removing dampness.