Objective:To study the treatment effect of a prescription of Chinese medicine which tonifies qi and removes toxicity on peripheral blood cytopenia and immune organs injury caused by 4 Gy ,60,Co γ ray in mice. MethodsA total of 120 Balb/c mice were randomly divided into two groups:the blank group and the irradiation group which were followed by whole-body exposure to 4 Gy ,60,Co γ ray. After irradiation,the irradiated mice were randomly divided into two groups:the model group and the Chinese medicine group. Mice of the blank and model groups were given deionized water by gavage once daily,while the others were administrated with the Chinese medicine. On the 14th,21th,35th,49th day of exposure,the mice were sacrificed respectively and the white blood cell (WBC),lymphocytes (LYMP),red blood cell (RBC),hemoglobin (HGB),blood platelets (PLT),thymus index,spleen index and spleen pathological section of each group were measured.  Results:Compared with the blank group,the peripheral blood cell amount and immune organ index decreased significantly and the pathological changes of the spleen were obvious in the model group on 14th day of exposure. At day 21 after irradiation,all kinds of blood cells in the model group gradually increased,the spleen index returned to normal level,the thymus index decreased again,the pathological changes of the spleen were alleviated,and the pathological sections showed that the hematopoiesis was obvious outside the red pulp. At day 35 after irradiation,thymus index of the model group returned to normal level, and the RBC,HGB,PLT of the Chinese medicine group regained to normal level. At day 49 after irradiation,the RBC,HGB,PLT of the model group just returned to normal level and the spleen structure was close to normal,while the WBC,LYMP were still lower than those of the blank group. The WBC,LYMP of the Chinese medicine group had returned to normal level. Compared with the model group,14 days after irradiation,the spleen index of the Chinese medicine group increased significantly,and the pathological changes of the spleen were slight; on the 35th day after irradiation,the RBC and HGB of the Chinese medicine group were significantly increased; and the WBC of the Chinese medicine group significantly increased on the 49th day after irradiation.  Conclusion:The prescription of Chinese medicine speeds up the recovery of peripheral blood cytopenia and immune organs injury after radiation in mice.