Objective:To observe the effects of Gutongling Prescription on the Blimp1/Bcl6 pathway in the mouse pain model of bone metastasis of lung cancer. Methods85 C57BL/6 mice were selected. Among, 17 mice were taken as the sham operation group, which treated with PBS solution. The pain model of bone metastasis of lung cancer was established in the other 68 mice by injecting Lewis cells suspension into the left hind tibia medullary cavity. Then the model mice were randomly divided into the model group, Chinese medicine group, western medicine group, Chinese and western medicine combination group, 17 mice in each group. The Chinese medicine group was treated with decoction of Gutongling Prescription by intragastric administration, the western medicine group was treated with zoledronic acid by intraperitoneal injection, and the Chinese and western medicine combination group was treated with decoction of Gutongling Prescription and zoledronic acid, with a course of 3 weeks. The osteoclasts of tibia in mice were detected by TRAP staining. The mRNA and protein expressions of B lymphocyte induced maturation protein1(Blimp1), B-cell lymphoma 6(Bcl6) and cathepsin K(Ctsk) were detected by RT-PCR and Western Blot.  Results:Compared with the model group, the number of TRAP positive cells was decreased remarkably in the Chinese medicine group, western medicine group and Chinese and western medicine combination group, and the mRNA and protein expressions of Blimp1 and Ctsk were reduced, while the mRNA and protein expressions of Bcl6 were increased, with statistically significant differences (P<0.05). Among the three drug treatment groups, the Chinese and western medicine combination group showed the least number of positive cells, the lowest mRNA and protein expressions of Blimp1 and Ctsk and the highest mRNA and protein expressions of Bcl6, with statistically significant differences (P<0.05).  Conclusion:Gutongling Prescription can suppress the osteoclast activation by inhibiting Blimp1/Bcl6 pathway, and thus alleviate the pain induced by bone metastasis of lung cancer.