Objective: To study the inhibitory effects and mechanism of Yiqi Fuzheng Compound Recipe combined with everolimus on xenograft of nude mice with triple negative breast cancer MDA-MB-468 cell line. MethodsThe triple negative breast cancer cell line MDA-MB-468 was cultured in vitro and the cell suspension was inoculated on the second breast pad of female BALB/C nude mice to establish a subcutaneous xenograft model. The successful modeling nude mice were randomly divided into the control group, Yiqi Fuzheng Compound Recipe (Chinese medicine compound recipe) group(40 mg/kg), everolimus group(2 mg/kg)and combination group, 6 mice in each group. Each group was treated with the corresponding drugs by intragastric administration for continuous 30 days. The volume and weight of transplanted tumor in each group were compared, and the tumor inhibition rate was calculated. The mRNA expressions of mTor, Akt, P70s6k and 4Ebp-1 in each group was detected by Real-time PCR. The protein expressions of Akt, p-Akt, m-TOR, p-mTOR, PTEN and p-4EBP-1 and p-P70S6K(as mTOR downstream substrates)were detected by Western blot.  Results:Compared with the Chinese medicine compound recipe group, the volume and weight of transplanted tumor in the combination group were decreased significantly(P<0.05,P<0.01), and the tumor inhibition rate was obviously increased. The mRNA expressions of mTor and P70s6k and the protein expressions of p-mTOR and P70S6K in the combination group were obviously lower than those in the Chinese medicine compound recipe group, and the protein expression of PTEN in the combination group was higher than that in the Chinese medicine compound recipe group(P<0.05).  Conclusion:The combination of Yiqi Fuzheng Compound Recipe and everolimus can inhibit the growth of tumor tissue in nude mice bearing MDA-MB-468 cell, and the effects are more significant compared with Yiqi Fuzheng Compound Recipe alone. The mechanism may be related to inhibiting the activities of p-mTOR, p-Akt, p-P70S6K and increasing the expressions of p-4EBP-1 and PTEN.