Objective:To investigate the effects of Shengjiang Powder on lipopolysaccharide (LPS) induced myocardial injury in septic rats via TLR-4/NF-κB signaling pathway. MethodsThe rat model of acute disease was established by intraperitoneal injection of LPS. The rats were randomly divided into the normal group (n=6), sepsis model group (n=6), Chinese medicine group (n=6) and inhibitor group (n=6). Except the normal group, rats in the other groups were intraperitoneally injected with lipopolysaccharide. The rats in the inhibitor group were injected with miR-146a inhibitor (0.2 μl/g) by intramyocardial administration, and the rats in the Chinese medicine group were treated with Shengjiang Powder by intragastric administration. After 48 hours, the myocardial tissue was collected and homogenized, the pathological changes of myocardial tissue sections were observed. The levels of interleukin (IL)-1, IL-6, tumor necrosis factor-α (TNF-α), and the mRNA activity levels of miRNA-146a, NF-κB, TNF-α, IL-6 and IL-1β were detected.  Results:Compared with the normal group, the levels of IL-1, IL-6, TLR4 and NF-κB were increased in the other groups (P<0.05). The TNF-α level in the model group and inhibitor group was higher than that in the normal group (P<0.05). The gene expressions of miR-146a, NF-κB, TNF-α, IL-6 and IL-1 in the model group were increased (P<0.01). The expressions of miR-146a and NF-κB in the Chinese medicine group were increased (P<0.05). The expressions of NF-κB, TNF-α, IL-6 and IL-1 in the inhibitor group were increased (P<0.01). Compared with the model group, the protein levels of TNF-α, TLR4 and NF-κB were decreased in the Chinese medicine group (P<0.05), and the expression of miR-146a was increased, while the gene expressions of NF-κB, TNF-α, IL-1 and IL-6 were decreased (P<0.05). Compared with the model group, the activities of IL-6, TNF-α, TLR4 and NF-κB in the inhibitor group were increased (P<0.01), and the expression of miR-146a was decreased (P<0.01), while the expressions of NF-κB, TNF-α, IL-6 and IL-1 were increased in the inhibitor group. Compared with the inhibitor group, the levels of IL-1, IL-6, TNF-α, TLR4 and NF-κB were decreased in the Chinese medicine group (P<0.01), and the gene expression of miR-146a was increased but the expressions of NF-κB, TNF-α, IL-6 and IL-1 were decreased (P<0.01).  Conclusion:Shengjiang Powder can reduce the myocardial damage in sepsis rats, and its mechanism may be associated with promotion on miR-146a expression, negative feedback regulation on TLR-4/NF-κB signaling pathway and inhibition on inflammatory reaction.