Objective:To establish the quality control method for Dongbo Tonglin Granule, which includes Huangbo (Phellodendri Chinensis Cortex), Rendongteng (Lonicerae Japonicae Caulis) and Bianxu (Polygoni Avicularis Herba). MethodsThe identifications of Huangbo, Rendongteng and Bianxu in this compound prescription were conducted by thin layer chromatography (TLC). The contents of berberine and chlorogenic acid were determined by high performance liquid chromatography (HPLC).  Results:The identification and separation of TLC was good and highly specific, and no interference was observed in the negative sample. The berberine showed a good linearity in the range of 0.108~2.16 μg (r=0.999 5). The average recovery was 101.61%(RSD=2.53%). The chlorogenic acid showed a good linearity in the range of 0.114~2.28 μg (r=0.999 6). The average recovery was 99.91%(RSD=2.32%).  Conclusion:This method is simple, accurate and reliable with strong specificity, and can be used for the quality control of Dongbo Tonglin Granule.