Objective:To observe the regulation effects of Changqi Drink on the levels or gene expressions of some brain-gut peptides in diarrhea-predominant irritable bowel syndrome(IBS-D) rats with syndrome of liver stagnation and spleen deficiency. Methods72 SPF male Wistar rats were randomly divided into the normal group,model group,Dicetel group and Changqi Drink group,18 rats in each group. Except the normal group,the IBS-D model with syndrome of liver stagnation and spleen deficiency was established by chronic restraint stress and folium sennae decoction in the other groups. Each group was treated with 0.9%NaCl solution,Dicetel and Changqi Drink decoction respectively. The serum level of 5-hoxytryptamine(5-HT) and the mRNA expressions of substance P(SP) and vasoactive intestinal peptide(VIP) in hypothalamus and colon tissue were detected.  Results:Changqi Drink could decrease the serum level of 5-HT and down-regulate the mRNA expressions of SP and VIP in hypothalamus and colon tissue(P<0.05).  Conclusion:Changqi Drink can regulate the dysfunction of brain-intestinal axis of IBS-D rats with syndrome of liver stagnation and spleen deficiency by decreasing the serum level of 5-HT and down-regulating the mRNA expressions of SP and VIP in hypothalamus and colon tissue.