新疆黑果小檗根皮、根木质部中阿魏酸提取工艺条件的优化比较研究

朱慧敏; 李莉; 李松雅; 闫琦

doi:10.16305/j.1007-1334.2018.06.025

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新疆黑果小檗根皮、根木质部中阿魏酸提取工艺条件的优化比较研究

实验研究 | 更新时间：2022-08-31

- 新疆黑果小檗根皮、根木质部中阿魏酸提取工艺条件的优化比较研究
- Comparative study on the optimization of extraction process conditions of ferulic acid in root bark and xylem of Xinjiang Berberis heteropda Schrenk
- 上海中医药杂志 2018年52卷第6期页码：87-94
- 作者机构：
  
  1. 新疆医科大学药学院,新疆,乌鲁木齐,830000
- 作者简介：
- 基金信息：
  
  国家自然科学基金资助项目(U1703108)
- DOI：10.16305/j.1007-1334.2018.06.025
  中图分类号：
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朱慧敏, 李莉, 李松雅, 等. 新疆黑果小檗根皮、根木质部中阿魏酸提取工艺条件的优化比较研究[J]. 上海中医药杂志, 2018,52(6):87-94.

ZHU Huimin, LI Li, LI Songya, et al. Comparative study on the optimization of extraction process conditions of ferulic acid in root bark and xylem of Xinjiang Berberis heteropda Schrenk[J]. Shanghai Journal of Traditional Chinese Medicine, 2018,52(6):87-94.
朱慧敏, 李莉, 李松雅, 等. 新疆黑果小檗根皮、根木质部中阿魏酸提取工艺条件的优化比较研究[J]. 上海中医药杂志, 2018,52(6):87-94. DOI： 10.16305/j.1007-1334.2018.06.025.

ZHU Huimin, LI Li, LI Songya, et al. Comparative study on the optimization of extraction process conditions of ferulic acid in root bark and xylem of Xinjiang Berberis heteropda Schrenk[J]. Shanghai Journal of Traditional Chinese Medicine, 2018,52(6):87-94. DOI： 10.16305/j.1007-1334.2018.06.025.

摘要

目的：采用 HPLC 法测定并对比新疆黑果小檗根皮与根木质部中阿魏酸的含量,并优化其提取工艺条件。方法：①通过不同溶剂提取黑果小檗根皮、根木质部,根据其含量测定结果选定最优提取溶剂。②在单因素试验结果基础上,采用响应曲面法优化阿魏酸的提取工艺。结果：①确定乙醇提物中阿魏酸含量最高。②确定最佳提取条件。平行试验3 次,新疆黑果小檗根皮、根木质部中的阿魏酸在提取溶剂为 60%乙醇,提取温度为 50 ℃,料液比为 1∶15,提取次数为 3 次的提取条件下提取效率最高,其中,根皮中阿魏酸提取量 0.71%(RSD=1.24%),根木质部中阿魏酸提取量 0.32%(RSD=1.51%)。结论：本提取工艺稳定可行,提取效率较高。

Abstract

Objective:To determinate and compare the content of ferulic acid in root bark and root xylem of Xinjiang Berberis heteropda Schrenk by HPLC,and optimize the extraction process conditions. Methods①The root bark and root xylem of Berberis heteropoda Schrenk were extracted with different solvents,and the optimum extraction solvent was selected according to the results of contents determination. ②Based on the results of single factor test,the extraction process of ferulic acid was optimized by response surface methodology. Results:①The content of ferulic acid in the ethanol extraction was the highest. ②The optimum extraction conditions were confirmed after three times parallel test. The extraction efficiency was maximum when the extraction solvent was 60% ethanol,the extraction temperature was 50 ℃,the liquid ratio was 1:15 and the times of extraction were 3. The extraction amount of ferulic acid was 0.71%(RSD=1.24%)in the root bark and 0.32%(RSD=1.51%)in the root xylem. Conclusion:This extraction process is stable and feasible with higher extraction efficiency.

关键词

黑果小檗根皮黑果小檗根木质部阿魏酸响应面法含量测定

Keywords

root bark of Berberis heteropda Schrenkroot xylem of Berberis heteropoda Schrenkferulic acidresponse surface methodologycontent determination

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