Objective:To analyze the pulse graph characteristics of non-disease population with different health status and age gradient based on self-developed pulse diagnosis instrument (PDA-1),and provide reference for the research on clinical application of Chinese medical pulse diagnosis. MethodsThe basic information and pulse graph of 1 720 non-disease subjects were collected. The health status of each case was evaluated by self-developed H20 questionnaire. The differences on pulse graphs and characteristic information were analyzed in healthy subjects with different age gradient,sub-healthy subjects with different age gradient and healthy and sub-healthy subjects with same age.  Results:①Among the 1 720 non-disease cases,there were 730 healthy cases and 990 sub-healthy cases. ②Compared with the healthy group,the value of h,5, was decreased significantly in the sub-healthy group (P,<,0.01),and the values of t,4,t,5,h,3,/h,1,h,4,/h,1,t,4,/t,5,w,1,/t and w,2,/t were increased significantly (P,<,0.05,P,<,0.01). ③In healthy subjects,there were no statistically significant differences on the pulse graph parameters between people of age from 40 to 49 year-old and those equal or more than 50 year-old gradient (P,>,0.05). There were statistically significant differences on the pulse graph parameters between people of age from 40 to 49 year-old and those less than 30 year-old or those of age from 30-39 year-old. To be specific,the value of h,5, was decreased significantly (P,<,0.01) and the values of h,3,h,4,t,4,h,3,/h,1,w,1,/t were increased significantly (P,<,0.05,P,<,0.01) in the pulse graph of subjects with age from 40 to 49 year-old. ④In sub-healthy subjects,with the increasing age,the value of h,5, was decreased significantly (P,<,0.01) and the values of t,1,t,4,t,4,/t,5, were significantly increased (P,<,0.05,P,<,0.01). ⑤For the comparison between the healthy and sub-healthy subjects with the same age gradient,in the age of ,<,30 and 30 to 39 year-old,the value of h5 was decreased significantly in sub-healthy subjects(P,<,0.05)；in the age of ,<,30 year-old,the values of t,4,h,3,/h,1,t,4,/t,5, and w,1,/t were increased significantly in sub-healthy subjects (P,<,0.05,P,<,0.01)；in the age of 30-39 year-old,the values of t,4, and h,4,/h,1, were significantly increased in sub-healthy subjects (P,<,0.05,P,<,0.01). There were no statistically significant differences on the pulse graph parameters between healthy and sub-healthy subjects at 40 to 49 year-old gradient and equal or more than 50 year-old gradient (P,>,0.05). Compared with the age of ,<,30 and 30-39 year-old,the value of h,5, was gradually decreased in the subjects with age of 40 to 49 and ≥50 year-old.  Conclusion:There are differences on the pulse graph between health and sub-healthy subjects,as well as subjects with same age gradient and different health status,which mainly present as the values of h,5,h,3,/h,1,w,1,/t and other indicators. The study results suggest that in sub-healthy subjects,the left ventricular ejection function and the arterial compliance are decreased,the arterial vascular elasticity is reduced,the peripheral resistance is increased and the pulse condition tends to be stringy pulse. The pulse graph can be considered as an objective basis for health status evaluation.