Objective:To observe the effects of Jiedu Huazhuo Decoction containing serum on the related proteins expression of PI3K/Akt signaling pathway in HER-2-positive breast cancer, and discuss the mechanism of Jiedu Huazhuo Decoction in the treatment of HER-2-positive breast cancer. MethodsFemale Wistar rats were randomly divided into the control group, Chinese medicine group, western medicine group and combination group. The drug containing serum was prepared from the rats treated with the corresponding drugs, and then the SK-BR-3 cells were treated with different drug containing serum. The protein expressions of HER-2, p-HER-2 and PI3K/Akt pathway-related proteins p-Akt, Bad and p-Bad were detected by Western blot.  Results:Western blot analysis showed that the grayscales of HER-2 and p-HER-2 in Chinese medicine group, western medicine group and combination group were obviously lower than those in the control group. The grayscales of HER-2, p-HER-2 and p-Akt in western medicine group and combination group were obviously decreased compared with the control group, and there were statistically significant differences on the expression levels of HER-2, p-HER-2 and p-Akt between the control group and western medicine group or combination group (P<0.05). The grayscales of Bad and p-Bad in Chinese medicine group, western medicine group and combination group were lower than those in the control group. There was no statistically significant difference on the total Bad expression among the groups (P>0.05). There was statistically significant difference on the p-Bad expression between the control group and western medicine group or combination group (P<0.05).  Conclusion:Jiedu Huazhuo Decoction can down-regulate the expression of related proteins in PI3K/Akt signaling pathway and exert anti-tumor effects combined with inhibitors through synergy and attenuation.