Objective:To investigate the influence of rutin on differentiation of 3T3-L1 preadipocytes. MethodsThe differentiation model of 3T3-L1 preadipocytes was established and oil red O staining was performed. Rosiglitazone was taken as the positive control drug. The effects of rutin on the differentiation of 3T3-L1 preadipocytes were observed,and the effect of rutin on the lipid content was detected at wavelength of 510 nm. The effects of rutin on the glucose absorption in differentiated adipocytes were determined by flow cytometry.  Results:At the concentrations of 0.5 and 1 μmol/L,rutin could promote the differentiation of 3T3-L1 preadipocytes,increase the lipid content,and promote the glucose absorption.  Conclusion:Rutin can promote the differentiation of 3T3-L1 preadipocytes and the glucose absorption in differentiated adipocytes.