Objective:To discuss the postponed effects of whole- and intermittent-way massage manipulation combined with treadmill training on denervated skeletal muscle atrophy. Methods40 SD rats were randomly divided into the normal control group,denervation group,whole-way massage group and intermittent-way massage group,10 rats in each group. Except the normal control group,the denervation model of sciatic nerve was established in the other groups. After modeling, no treatments were performed for 3 weeks. The whole-way massage group was treated with pinching manipulation and treadmill training once a day for 2 months,and the intermittent-way massage group was treated with pinching manipulation and treadmill training once a day for one month and then rested for one month. After intervention,the gastrocnemius wet weight ratio was detected,and the positive areas of cytokines including insulin-like growth factor-I(IGF-I),basic fibroblast growth factor(bFGF)and myogenic differentiation factor(MyoD)were determined.  Results:Compared with the whole-way massage group,the levels of cytokines were obviously increased in the intermittent-way massage group(P<0.05),and the gastrocnemius wet weight ratio was obviously decreased(P<0.05).  Conclusion:The whole-way massage manipulation combined with treadmill training can delay the denervated skeletal muscle atrophy in a certain extent.