Objective:To explore the effects of Qingre Lishi Recipe for external application (Rhizoma Polygoni Cuspidati,Herba Taraxaci,Radix Sophorae Flavescentis,Cortex Phellodendri) and polydatin on perianal wound healing in SD rats. MethodsSD rat model of perianal wound was established. The rats were randomly divided into the Qingre Lishi Recipe group,polydatin group,normal saline group,45 rats in each group. Interventions were given in the corresponding groups. 3,7,11 days after operation,the wound capillary permeability was observed by evans blue staining,the wound exudation,inflammation and healing were observed by naked eye and electron microscope,the tight junction of vascular endothelial cells and synthesis and distribution of collagen fibrils were observed by electron microscope.  Results:On 3rd day after modeling,there was no difference on the leakage of evans blue between the Qingre Lishi group and polydatin group(P>0.05),but the leakage in these two groups was less than that in the normal saline group(P<0.05). On 7th and 11th day,there were no differences on the leakage of evans blue among the groups(P>0.05). The observation by naked eye showed that there were no differences on the wound exudation among the groups on 3rd,7th,11th day after modeling(P>0.0167). On 3rd,7th day after operation,the granulation hyperplasia and epithelial migration in the Qingre Lishi group were better than those in the normal saline group(P<0.0167),but there were no statistically significant differences on the granulation hyperplasia and epithelial migration between the Qingre Lishi group and polydatin group(P>0.0167)as well as the polydatin group and normal saline group(P>0.0167). On 11th day,there were no differences on the wound healing among the groups(P>0.0167). The light microscopy results showed that there was no obvious edema of granulation tissue in the Qingre Lishi group,the new capillaries were abundant and the fibroblasts arranged in order. The light microscopy observation in the polydatin group was similar to that in the Qingre Lishi group,and the wound healing in these two groups was better than that in the normal saline group. The electron microscopy results showed that there were tight junction of vascular endothelial cells and close fitting with adjacent cells in the Qingre Lishi group,the collagen fibrils distribution was compact and regular,the fiber bundle arranged closely.  Conclusion:Qingre Lishi Recipe and polydatin can reduce the permeability of blood vessel and control the inflammatory exudation of wound,but Qingre Lishi Recipe can more effectively promote the epithelial migration and accelerate the regeneration of various components in granulation tissue.