Objective:To preliminarily discuss the mechanism of Liushen Pill on reversing doxorubicin resistant human colon cancer cell line Lovo/Dox. MethodsThe control group,doxorubicin group,Liushen Pill group and combination group were divided. The effects of Liushen Pill on drug-resistant human colon cancer cell lines (Lovo/Dox,HCT116/L-OHP,Caco,2,/Dox)were detected by Cell Counting Kit-8(CCK-8). The expressions of P-gp, BCRP and related apoptosis proteins caspase-3, caspase-9, Cytochrome C and tumor suppressor protein p53 in human colon cancer cell line Lovo/Dox were determined by Western blot. The fluorescence expression of P-gp was detected by immunofluorescence assay.  Results:After the intervention of Liushen Pill for 24 hours,the CCK-8 showed that Liushen Pill could inhibit the activities of the three drug-resistant human colon cancer cell lines,among the inhibiting effects on doxorubicin resistant human colon cancer cell line Lovo/Dox were most significant. The treatment of Liushen Pill combined with doxorubicin could up-regulate the protein expression of caspase-3,caspase-9 and p53 and decrease the protein and fluorescence expression of P-gp.  Conclusion:Liushen Pill can reverse the expression of P-gp in doxorubicin resistant human colon cancer cell line Lovo/Dox, which may be related to the activation of the related apoptosis pathway and tumor suppressor protein.