Objective:To observe the effects of Gutongling Prescription on the expression of monocyte chemoattractant protein-1(MCP-1) and nerve growth factor(NGF) in the spinal cord of mice with bone metastasis of lung cancer model,and discuss its potential mechanism on the treatment of bone cancer pain. MethodsThe mice model of lung cancer with bone metastasis was established by the injection of Lewis lung cancer cells into the bone marrow cavity of left leg tibia. 50 mice were randomly divided into five groups:sham-operation group, model group, Chinese medicine group, western medicine group, Chinese and western medicine combination group,10 in each mice. The Chinese medicine group was treated with decoction of Gutongling Prescription for intragastric administration,the western medicine group was treated with zoledronic acid for intraperitoneal injection,the combination group was treated with Gutongling Prescription and zoledronic acid. Before modeling and 6(7),13(14),20(21)days after modeling,the changes of paw withdrawal mechanical threshold(PWMT) and paw withdrawal thermal latency(PWTL) were observed by Von Frey hairs and heat stimulation test. The protein expressions of MCP-1 and NGF in the spinal cord were detected by enzyme linked immunosorbent assay (ELISA).  Results: 3 weeks after modeling, the PWMT and PWTL of the three treatment groups were obviously higher than those of the model group(P<0.05),the PWMT of the combination group was higher than that of the Chinese medicine group and western medicine group,with statistically significant difference(P<0.05). Compared with the model group,the protein expression of MCP-1 in all the three treatment groups was obviously decreased(P<0.05),and the NGF expression was significantly increased(P<0.05).  Conclusion: Gutongling Prescription can suppress the central sensitization by decreasing the protein expression of MCP-1 in the spinal cord,and repair the damaged neurons by promoting the protein expression of NGF,and consequently exert the analgesic effect.