Objective:To investigate the anti-tumor effect of Qingre Jiedu(QRJD) Formula on transplanted hepatocellular carcinoma in mice and the influence on chemokine. MethodsMice models of CBRH-7919 orthotopic hepatocellular carcinoma were established,and the mice were randomly divided into the control group and Chinese medicine groups with low-,mid- and high-dose. The anti-tumor effect of QRJD Formula was observed. The differential genes (with at least 3 folds) among different dose groups were screened by the chemokine chip,and then the differential genes were verified by immunohistochemistry and Western blotting.  Results:① QRJD Formula with different doses all showed anti-tumor effect in varying degree(P<0.01 compared with the control group),comparison among groups,the anti-tumor effect of high-dose group was more significant(P<0.01).② CXCL1, CXCL2, CXCL3, CXCR2 and IL-1β were screened as differential gene by the chemokine microarray detection,among,the gene expression of CXCL1, CXCL2, CXCL3 and IL-1β were down-regulated in the Chinese medicine intervention group and the gene expression of CXCR2 was up-regulated,which more significant in the high-dose group (P<0.05).③Above differential gene were verified by immunohistochemistry and Western blotting on the protein levels.  Conclusion:QRJD Formula could significantly down-regulate the expression of chemotactic factors CXCL1, CXCL2, CXCL3 and IL-1β and up-regulate the chemotactic factor receptor CXCR2 in mice with transplanted hepatocellular carcinoma,which may be related to its mechanism on anti-tumor.