灵猫方对HepG2.2.15和HepAD38细胞La蛋白表达的影响

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灵猫方对HepG2.2.15和HepAD38细胞La蛋白表达的影响

实验研究 | 更新时间：2022-08-31

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- 灵猫方对HepG2.2.15和HepAD38细胞La蛋白表达的影响
- Effect of Lingmao Formula on the La protein expression in HepG2.2.15 and HepAD38 cells
- 上海中医药杂志 2017年51卷第1期页码：88-91
- 作者机构：
  
  1. 上海中医药大学附属曙光医院肝病科,上海,201203
  2. 上海中医药大学附属曙光医院细胞免疫实验室,上海,201203
- 作者简介：
- 基金信息：
  
  国家自然科学基金项目(81403351);上海市自然基金资助项目(14ZR1441900);国家 “艾滋病和病毒性肝炎等重大传染病防治”科技重大专项(2012ZX10005004-002);上海市中医药事业发展三年行动计划项目(ZY3-LCPT-1-1001,ZY3-CCCX-3-3009,ZY3-CCCX-3-3027,ZY3-CCCX-3-3029);上海申康市级医院新兴前沿技术联合攻关项目(SHDC12016121);上海市科委生物引导项目(16401931300);上海市科委科技支撑项目(16401970600);上海市科委扬帆计划项目(15YF1412300,14YF1411600);上海中医药大学预算内项目(2014YSN39)
- DOI：
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朱晓骏, 张鑫, 李曼, 等. 灵猫方对HepG2.2.15和HepAD38细胞La蛋白表达的影响[J]. 上海中医药杂志, 2017,51(1):88-91.

ZHU Xiao-jun, ZHANG Xin, LI Man, et al. Effect of Lingmao Formula on the La protein expression in HepG2.2.15 and HepAD38 cells[J]. Shanghai Journal of Traditional Chinese Medicine, 2017,51(1):88-91.
朱晓骏, 张鑫, 李曼, 等. 灵猫方对HepG2.2.15和HepAD38细胞La蛋白表达的影响[J]. 上海中医药杂志, 2017,51(1):88-91. DOI：

ZHU Xiao-jun, ZHANG Xin, LI Man, et al. Effect of Lingmao Formula on the La protein expression in HepG2.2.15 and HepAD38 cells[J]. Shanghai Journal of Traditional Chinese Medicine, 2017,51(1):88-91. DOI：

摘要

目的：研究灵猫方对HepG2.2.15和HepAD38细胞cccDNA、pgRNA、sRNA和La-mRNA抑制作用,探讨灵猫方抗乙肝病毒(HBV)的作用机制。方法：制备灵猫方水提物,500 mg/L和1 000 mg/L灵猫方水提物分别干预HepG2.2.15和HepAD38细胞,以0.9%NaCl溶液作为空白对照组,6 d后收集培养液上清,ELISA法检测HBsAg和HBeAg水平；收集细胞提取总RNA,RT-PCR法检测细胞内cccDNA、pgRNA、sRNA和La mRNA表达水平。结果：与空白对照组比较,灵猫方组的HepG2.2.15和HepAD38细胞的HBsAg和HBeAg分泌水平明显降低,细胞内cccDNA、pgRNA、sRNA和La mRNA表达水平明显降低(P< 0.01)。结论：灵猫方可能通过抑制HepG2.2.15和HepAD38细胞内La蛋白的表达而促进pgRNA、sRNA和cccDNA的降解,从而抑制HBV的复制。

Abstract

Objective:To study the inhibitive effects of Lingmao Formula on cccDNA, pgRNA, sRNA and La-mRNA in HepG2.2.15 and HepAD38 cells, and discuss its mechanism on anti-hepatitis B virus(HBV). MethodsThe aqueous extract of Lingmao Formula was prepared. HepG2.2.15 and HepAD38 cells were intervened by the aqueous extract of Lingmao Formula at concentration of 500 and 1 000 mg/L respectively,the blank control group was treated with normal saline. The supernatant of culture medium was collected after 6 days and the levels of HBsAg and HBeAg were detected by ELISA. The cells were collected and the total RNA was extracted,the expression levels of cccDNA, pgRNA, sRNA and La mRNA were detected by RT-PCR. Results:Compared with the blank control group, the secretion levels of HBsAg and HBeAg in HepG2.2.15 and HepAD38 cells of the Lingmao Formula group were significantly decreased, the expression levels of intracellular cccDNA, pgRNA, sRNA and La mRNA were significantly decreased (P< 0.01). Conclusion:Lingmao Formula may promote the degradation of pgRNA, sRNA and cccDNA by inhibiting La protein expression in HepG2.2.15 and HepAD38 cells, and result in the inhibition of HBV replication.

关键词

灵猫方HepG2.2.15HepAD38La蛋白pgRNAcccDNAsRNA

Keywords

Lingmao FormulaHepG2.2.15HepAD38La proteinpgRNAcccDNAsRNA

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