Objective:To observe the effects of electroacupuncture(EA) on depressive-like behavior and c-fos protein expression of habenular nucleus in the WKY rat model. Methods24 male WKY rats were randomly divided into the EA, sham and model groups,8 in each group.8 Wistar male rats were set as normal group. The behaviors were evaluated by open field test, sucrose preference test and force swimming test, the expression of c-fos was detected by immunofluorescence.  Results:Compared with the control group , the behaviors of open field test and Sucrose preference test in model group decreased significantly (P<0.05),and the immobile time of force swimming test and c-fos expression of habenular nucleus increased significantly (P<0.05). Compared with sham group , the behaviors of open field test and Sucrose preference test in EA group increased significantly (P<0.05),and the immobile time of force swimming test and c-fos expression of habenular nucleus decreased significantly (P<0.05).  Conclusion:Electroacupuncture cauld improve the depressive- like behaviors of WKY rats and decrease the expression of c-fos protein in habenular nucleus. It may be one of the mechanisms of electroacupuncture on depression.