Objective:To investigate the anti-hyperglycemic effects of the compatibility of ginsenoside Re and berberine in different ratio on type 2 diabetes. MethodsThe different ratio of ginsenoside Re and berberine (2∶0, 16∶1, 1∶2, 8∶4, 0∶8, 4∶16) were matched by uniform design method. The hyperglycemia model in mice was induced by starch(6 mg/g), and the type 2 diabetes model in rats was induced by STZ(150 μg/g)and ALX(200 μg/g). The effects of different ratio of ginsenoside Re and berberine(100 μg/g) on blood sugar were observed.  Results:postprandial blood glucose in starch induced mice treated with the ratio of ginsenoside Re and berberine at 1∶2(100 μg/g), 8∶4(100 μg/g), 4∶16(100 μg/g) significantly reduced(P<0.05 or P<0.01). the blood glucose level in STZ induced rats treated with the ratio of ginsenoside Re and berberine at 8∶4(100 μg/g)and 4∶16(100 μg/g) significantly inhibited(P<0.05 or P<0.01). The ratio of Ginsenoside Re and berberine at 4∶16 also showed significantly reverse effect on ALX-induced hyperglycemia in rats (P<0.01), and its hypoglycemic effect was similar to phenformin(100 μg/g). In addition, among the different ratio in the STZ and ALX induced models, the inhibition of blood glucose in the ratio of ginsenoside Re and berberine at 4∶16 was more effective than other groups(P<0.05).  Conclusion:The ratio of ginsenoside Re and berberine at 4∶16(100 μg/g) showed significantly anti-hyperglycemic effect.