Objective:To study the effect of different doses of JianYao-MiGu tablet on mice bone formation and BMP7 expression of bone marrow mesemnchymal stem cells (BMSCs) in mice during the osteogenetic differentiation process. Methods Sixty 1-month-old Kunming mice, male thirty and female thirty, were randomly divided into four groups of high-dose, middle-dose, low-dose, and saline group. All the mice were treated intragastrically and sacrificed after 8 weeks. The fourth lumbar vertebra (L4) was scanned by Micro-CT, The BMP-7 expression of each group were observed using immunohistochemical staining. Primary bone marrow-derived mesenchymal stem cells (BMSCs) were isolated and cultured in each group. Alkaline phosphatase (ALP) staining was performed to observe the osteogenic differentiation, and real-time quantitative PCR analysis was performed to observe the mRNA expression of BMP-7.  Results: Compared to normal saline group, high-dose group improved mice vertebral three-dimensional structure, increased the activity of ALP, promoted the mRNA and protein expression of BMP-7 expression.  Conclusion:High-dose JianYao-MiGu tablet could promote the expression of ALP in BMSCs and osteogenic differentiation, increase the expression of BMP-7, thus as to promote bone formation.